Recombinant Human ASAH2 protein, His-tagged

• Cat.No.: ASAH2-115H
• Product Overview: Recombinant Human ASAH2 protein(Q9NR71)(610-780aa), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 25.2 kDa
• Protein length: 610-780aa
• AA Sequence: FRNLAKAIATDTVANLSRGPEPPFF KQLIVPLIPSIVDRAPKGRTFGDVL QPAKPEYRVGEVAEVIFVGANPKNS VQNQTHQTFLTVEKYEATSTSWQIV CNDASWETRFYWHKGLLGLSNATVE WHIPDTAQPGIYRIRYFGHNRKQDI LKPAVILSFEGTSPAFEVVTI
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 [ Homo sapiens ]
• Official Symbol: ASAH2
• Synonyms: ASAH2; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; neutral ceramidase; mitochondrial ceramidase; non-lysosomal ceramidase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; N-acylsphingosine amidohydrolase 2; HNAC1; BCDase; LCDase; NCDase; N-CDase
• Gene ID: 56624
• mRNA Refseq: NM_001143974
• Protein Refseq: NP_001137446
• MIM: 611202
• UniProt ID: Q9NR71

Reviews

04/04/2019

It reliably and accurately detects and quantifies target molecules, allowing for precise measurement of analytes in my samples.

02/25/2019

I highly recommend the ASAH2 Protein for its outstanding performance in ELISA assays.

11/22/2016

The sensitivity and specificity of the ASAH2 Protein have consistently delivered robust and consistent results, enhancing the efficiency and accuracy of my experimental workflows.

Q&As

Can ASAH2 activity be regulated by post-translational modifications?

Post-translational modifications, such as phosphorylation, may impact ASAH2 activity, although further research is needed for a comprehensive understanding.

Are there any known inhibitors of ASAH2?

While specific inhibitors for ASAH2 are not yet available, researchers are actively exploring potential compounds that can modulate its activity.

In which cellular compartments is ASAH2 found?

ASAH2 is primarily localized in the lysosomes, where it carries out the ceramide hydrolysis process.

What are the consequences of ASAH2 deficiency?

Deficiency in ASAH2 activity can lead to the accumulation of ceramides, which may disrupt cellular signaling and contribute to disease development.

How is ASAH2 gene expression regulated?

ASAH2 gene expression can be regulated by various factors, including transcription factors, epigenetic modifications, and signaling pathways.

Are there any diseases associated with ASAH2?

Mutations in the ASAH2 gene have been associated with a rare form of hereditary sensory and autonomic neuropathy (HSAN) called HSAN1C.

Is ASAH2 ubiquitously expressed in all tissues?

Yes, ASAH2 is ubiquitously expressed in various tissues throughout the body.