Recombinant Human ASAM protein(Met1-Met233), His-tagged

• Cat.No.: ASAM-587H
• Product Overview: Recombinant Human ASAM (NP_079045.1)(Met 1-Met 233) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Met233
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human ASAM comprises 227 amino acids and predicts a molecular mass of 25.8 kDa. It migrates as an approximately 32-35 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

08/31/2020

It has consistently demonstrated high sensitivity and accuracy in detecting and quantifying target molecules, allowing for precise and reliable measurement of analytes in my experimental samples.

03/12/2020

Its versatility and reliability make it a top choice for researchers in various fields.

10/16/2017

The ASAM Protein's adaptability has significantly expanded the scope of my investigations and broadened the possibilities for my research.

Q&As

Are there any known genetic variations or polymorphisms in ASAM?

The presence of genetic variations or polymorphisms in ASAM has not been extensively investigated, and further research is required to ascertain this.

Does ASAM affect the opening or closing of MiniK channels?

ASAM primarily affects the opening of MiniK channels, regulating their overall activity and the efflux of potassium ions.

How does ASAM modulate sodium and MiniK channels?

ASAM binds to sodium and MiniK channels, altering their activity and influencing the flow of ions through these channels.

Is ASAM associated with any specific diseases?

Currently, there are no reported disease associations specific to ASAM. More research is needed to determine its potential involvement in diseases.

Is ASAM expressed in all cell types?

ASAM is not universally expressed in all cell types, and its expression pattern may vary depending on the tissue or cell type.

What are the cellular consequences of ASAM's modulation of sodium and MiniK channels?

ASAM's modulation of these channels can impact the excitability and electrical properties of cells, influencing their behavior and function.

Does ASAM affect the opening or closing of sodium channels?

ASAM can affect both the opening and closing of sodium channels, leading to changes in their overall activity.