Recombinant Human ASB7 293 Cell Lysate

• Cat.No.: ASB7-8660HCL

Specification

• Description: Antigen standard for ankyrin repeat and SOCS box-containing 7 (ASB7), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ASB7 ankyrin repeat and SOCS box containing 7 [ Homo sapiens ]
• Official Symbol: ASB7
• Synonyms: ASB7; ankyrin repeat and SOCS box containing 7; ankyrin repeat and SOCS box protein 7; ASB-7; ankyrin repeat and SOCS box-containing 7; FLJ22551; FLJ25192
• Gene ID: 140460
• mRNA Refseq: NM_024708
• Protein Refseq: NP_078984
• UniProt ID: Q9H672
• Chromosome Location: 15q26.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem

Reviews

10/04/2016

Should I encounter any challenges or require technical assistance, the manufacturer provides prompt and knowledgeable support.

05/29/2016

Their expertise in protein production and purification ensures the delivery of a high-quality product that consistently performs well in my experiments.

05/27/2016

the manufacturer's commitment to quality control and rigorous testing guarantees the reliability and reproducibility of the ASB7 protein, which is essential for obtaining accurate and consistent results.

Q&As

Are there any known therapeutic implications or potential targets involving ASB7?

Currently, there are no known therapeutic implications specifically related to ASB7. However, exploring its functions and interactions may reveal potential therapeutic targets for certain diseases or conditions.

Are there any known genetic variants or mutations in the ASB7 gene?

Although ASB7 genetic variants may exist, they have not been extensively studied. Further research is required to identify and understand any potential genetic changes in ASB7.

Is the expression of ASB7 regulated by any specific factors or conditions?

The specific factors or conditions that regulate ASB7 expression remain largely unknown. Investigating its expression patterns under different conditions could provide insights into its regulatory mechanisms.

Is there any evidence to suggest a role for ASB7 in cellular metabolism or energy homeostasis?

The involvement of ASB7 in cellular metabolism or energy homeostasis is not well-established. Further research is needed to determine if ASB7 plays a role in these processes.