Recombinant Human ASB8 293 Cell Lysate

• Cat.No.: ASB8-8659HCL

Specification

• Description: Antigen standard for ankyrin repeat and SOCS box-containing 8 (ASB8) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ASB8 ankyrin repeat and SOCS box containing 8 [ Homo sapiens ]
• Official Symbol: ASB8
• Synonyms: ASB8; ankyrin repeat and SOCS box containing 8; ankyrin repeat and SOCS box protein 8; FLJ21255; MGC5540; ASB-8; ankyrin repeat and SOCS box-containing 8; PP14212
• Gene ID: 140461
• mRNA Refseq: NM_024095
• Protein Refseq: NP_077000
• UniProt ID: Q9H765
• Chromosome Location: 12q13.12
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem

Reviews

10/17/2022

They are readily available to address any concerns or questions and supply valuable resources to assist in experimental design and optimization.

07/25/2020

the manufacturer offers a wide range of ASB8 protein variants, including recombinant or native forms, as well as different conjugates and modifications.

04/07/2018

As a researcher using ASB8 protein, I have found numerous advantages in its application during trials for various experimental purposes.

Q&As

Is ASB8 expressed in specific tissues or cell types?

ASB8 is known to be expressed in various tissues, including skeletal muscle, heart, and liver. It may have tissue-specific expression patterns, but more research is needed to fully understand its expression profile.

Are there any disease associations with ASB8?

Currently, there are no known diseases directly associated with ASB8. Further investigations could reveal potential associations with certain diseases or disorders.

Is ASB8 regulated by any signaling pathways or stimuli?

The regulation of ASB8 by signaling pathways or stimuli has not been extensively studied. Future research could explore any potential regulation of ASB8 expression or activity.

Does ASB8 have any known interacting partners?

The specific interacting partners of ASB8 have not been extensively characterized. Further studies are required to identify proteins or molecules that interact with ASB8.

Does ASB8 play a role in cellular processes such as cell proliferation or differentiation?

The role of ASB8 in cellular processes such as cell proliferation or differentiation is not well-defined. Additional studies are needed to determine if ASB8 is involved in these processes.