Recombinant Human ATP2B4 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ATP2B4-6544H
• Product Overview: ATP2B4 MS Standard C13 and N15-labeled recombinant protein (NP_001675) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 133.8 kDa
• AA Sequence: MTNPSDRVLPANSMAESREGDFGCT VMELRKLMELRSRDALTQINVHYGG VQNLCSRLKTSPVEGLSGNPADLEK RRQVFGHNVIPPKKPKTFLELVWEA LQDVTLIILEIAAIISLVLSFYRPA GEENELCGQVATTPEDENEAQAGWI EGAAILFSVIIVVLVTAFNDWSKEK QFRGLQCRIEQEQKFSIIRNGQLIQ LPVAEIVVGDIAQVKYGDLLPADGI LIQGNDLKIDESSLTGESDHVKKSL DKDPMLLSGTHVMEGSGRMVVTAVG VNSQTGIILTLLGVNEDDEGEKKKK GKKQGVPENRNKAKTQDGVALEIQP LNSQEGIDNEEKDKKAVKVPKKEKS VLQGKLTRLAVQIGKAGLLMSALTV FILILYFVIDNFVINRRPWLPECTP IYIQYFVKFFIIGITVLVVAVPEGL PLAVTISLAYSVKKMMKDNNLVRHL DACETMGNATAICSDKTGTLTMNRM TVVQAYIGGIHYRQIPSPDVFLPKV LDLIVNGISINSAYTSKILPPEKEG GLPRQVGNKTECALLGFVTDLKQDY QAVRNEVPEEKLYKVYTFNSVRKSM STVIRNPNGGFRMYSKGASEIILRK CNRILDRKGEAVPFKNKDRDDMVRT VIEPMACDGLRTICIAYRDFDDTEP SWDNENEILTELTCIAVVGIEDPVR PEVPDAIAKCKQAGITVRMVTGDNI NTARAIATKCGILTPGDDFLCLEGK EFNRLIRNEKGEVEQEKLDKIWPKL RVLARSSPTDKHTLVKGIIDSTVGE HRQVVAVTGDGTNDGPALKKADVGF AMGIAGTDVAKEASDIILTDDNFTS IVKAVMWGRNVYDSISKFLQFQLTV NVVAVIVAFTGACITQDSPLKAVQM LWVNLIMDTFASLALATEPPTESLL KRRPYGRNKPLISRTMMKNILGHAF YQLIVIFILVFAGEKFFDIDSGRKA PLHSPPSQHYTIVFNTFVLMQLFNE INSRKIHGEKNVFSGIYRNIIFCSV VLGTFICQIFIVEFGGKPFSCTSLS LSQWLWCLFIGIGELLWGQFISAIP TRSLKFLKEAGHGTTKEEITKDAEG LDEIDHAEMELRRGQILWFRGLNRI QTQIKVVKAFHSSLHESIQKPYNQK SIHSFMTHPEFAIEEELPRTPLLDE EEEENPDKASKFGTRVLLLDGEVTP YANTNNNAVDCNQVQLPQSDSSLQS LETSVTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ATP2B4 ATPase plasma membrane Ca2+ transporting 4 [ Homo sapiens (human) ]
• Official Symbol: ATP2B4
• Synonyms: ATP2B4; ATPase, Ca++ transporting, plasma membrane 4; ATP2B2, matrix remodelling associated 1, MXRA1; plasma membrane calcium-transporting ATPase 4; plasma membrane calcium transporting ATPase 4; PMCA4; sarcolemmal calcium pump; matrix-remodeling-associated protein 1; MXRA1; ATP2B2; PMCA4b; PMCA4x; DKFZp686M088; DKFZp686G08106
• Gene ID: 493
• mRNA Refseq: NM_001684
• Protein Refseq: NP_001675
• MIM: 108732
• UniProt ID: P23634

Reviews

06/10/2021

Because it is not easy to be contaminated, it is stored again.

10/13/2020

The protein's activity is truly remarkable, able to catalyze reactions even under extreme conditions.

05/14/2020

ATP2B4's stability is truly remarkable, able to maintain its activity even under extreme conditions.

Q&As

Are mutations in ATP2B4 associated with rare genetic diseases?

ATP2B4 gene mutation may be related to some hereditary diseases such as acoustic neuroma and lung cancer.

What is the biological function of ATP2B4?

This is a calcium transporter, which is mainly responsible for transporting intracellular calcium to the outside of the cell, thereby maintaining the intracellular and extracellular calcium balance.

How does the protein structure and function of ATP2B4 differ from other atpase B family members?

There are several differences in protein structure and function between ATP2B4 and other atpase B family members, mainly depending on how they are expressed and regulated in different tissues and cells.

What are the pathways of ATP2B4 protein production?

The production pathway of ATP2B4 protein mainly includes multiple biological processes such as gene transcription, RNA splicing, protein translation and subsequent modification.

What diseases are associated with ATP2B4 expression?

The expression of ATP2B4 may be related to the occurrence and development of diabetes, obesity, cardiovascular disease, liver disease and other diseases.

Is ATP2B4 associated with cardiovascular disease?

The relationship between ATP2B4 and cardiovascular disease may be achieved through several mechanisms such as regulating cardiomyocyte contraction and vascular smooth muscle cell contraction.