Recombinant Human ATP4B protein, GST-tagged

• Cat.No.: ATP4B-2943H
• Product Overview: Recombinant Human ATP4B(1 a.a. - 291 a.a.) fused with GST tag at N-terminal was expressed in Wheat Germ.

Specification

• Description: The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
• Molecular Mass: 57.75 kDa
• AA Sequence: MAALQEKKTCGQRMEEFQRYCWNPD TGQMLGRTLSRWVWISLYYVAFYVV MTGLFALCLYVLMQTVDPYTPDYQD QLRSPGVTLRPDVYGEKGLEIVYNV SDNRTWADLTQTLHAFLAGYSPAAQ EDSINCTSEQYFFQESFRAPNHTKF SCKFTADMLQNCSGLADPNFGFEEG KPCFIIKMNRIVKFLPSNGSAPRVD CAFLDQPRELGQPLQVKYYPPNGTF SLHYFPYYGKKAQPHYSNPLVAAKL LNIPRNAEVAIVCKVMAEHVTFNNP HDPYEGKVEFKLKIEK
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.

Gene Information

• Gene Name: ATP4B ATPase, H+/K+ exchanging, beta polypeptide [ Homo sapiens ]
• Official Symbol: ATP4B
• Synonyms: ATP4B; ATPase, H+/K+ exchanging, beta polypeptide; potassium-transporting ATPase subunit beta; ATP6B; proton pump beta chain; gastric H+/K+ ATPase beta subunit; gastric H(+)/K(+) ATPase subunit beta; gastric hydrogen-potassium ATPase, beta; potassium-transporting ATPase beta chain; ATPase, H+/K+ transporting, beta polypeptide
• Gene ID: 496
• mRNA Refseq: NM_000705
• Protein Refseq: NP_000696
• MIM: 137217
• UniProt ID: P51164
• Chromosome Location: 13q34
• Pathway: Collecting duct acid secretion, organism-specific biosystem; Collecting duct acid secretion, conserved biosystem; Gastric acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem; Ion channel transport, organism-specific biosystem; Ion transport by P-type ATPases, organism-specific biosystem; Oxidative phosphorylation, organism-specific biosystem
• Function: hydrogen:potassium-exchanging ATPase activity; sodium:potassium-exchanging ATPase activity

Reviews

12/22/2022

Their wide range of products can meet our needs in different research areas.

10/13/2021

ATP4B were highly reproducible and showed good process control and product quality.

Q&As

Are there any studies on drugs that regulate gastric acid secretion by ATP4B?

Yes, several studies have been devoted to the development of drugs that can modulate ATP4B activity for the regulation of gastric acid secretion and the treatment of related gastric diseases.

What is the regulatory mechanism of ATP4B in gastric acid secretion?

ATP4B regulates gastric acid secretion through ATP binding and ion pump transport, and the specific mechanism involves the regulation of a variety of signaling pathways and hormones.

Is the abnormal expression of ATP4B valuable in the diagnosis and prognosis of gastric-related diseases?

The abnormal expression of ATP4B can be used as an important indicator for the diagnosis and prognosis of some gastric-related diseases, but it needs to be comprehensively analyzed in combination with other clinical and molecular biological results.

What other proteins does ATP4B interact with?

Interacts with other proteins in the Parietal cells of the gastric mucosa, such as those associated with the parietal cells of the gastric mucosa, to jointly regulate the production and secretion of gastric acid.

What is the mutation mechanism of ATP4B?

Mutations in ATP4B may lead to abnormal changes in its intracellular localization and function, and then affect the normal secretion of gastric acid.

Is the polymorphism of ATP4B gene associated with abnormal gastric acid?

Yes, polymorphic variations in the ATP4B gene may be associated with the susceptibility and pathogenesis of gastric acid abnormalities, such as hyperacidity or hypoacidity.