Recombinant Human ATP5A1 293 Cell Lysate

• Cat.No.: ATP5A1-8606HCL

Specification

• Description: Antigen standard for ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle (ATP5A1), nuclear gene encoding mitochondrial protein, transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ATP5A1 ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle [ Homo sapiens ]
• Official Symbol: ATP5A1
• Synonyms: ATP5A1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle , ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non cardiac muscle like 2 , ATP5AL2, ATPM; ATP synthase subunit alpha, mitochondrial; ATP5A; hATP1; OMR; ORM; ATP sythase (F1-ATPase) alpha subunit; ATP synthase alpha chain, mitochondrial; mitochondrial ATP synthetase, oligomycin-resistant; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATPM; MOM2; ATP5AL2
• Gene ID: 498
• mRNA Refseq: NM_001001935
• Protein Refseq: NP_001001935
• MIM: 164360
• UniProt ID: P25705
• Chromosome Location: 18q21
• Pathway: Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Electron Transport Chain, organism-specific biosystem; F-type ATPase, eukaryotes, organism-specific biosystem; Formation of ATP by chemiosmotic coupling, organism-specific biosystem; Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem
• Function: ADP binding; ATP binding; contributes_to ATPase activity; MHC class I protein binding; eukaryotic cell surface binding; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances; nucleotide binding; protein binding; proton-transporting ATPase activity, rotational mechanism; transmembrane transporter activity

Reviews

08/04/2020

It is not easy to be contaminated, and then stored.

11/29/2019

The reproducibility of ATP5A1 is very good, with very consistent results in every experiment or application, benefiting from its high degree of stability and reproducibility.

05/30/2019

Using ATP5A1 as a catalyst, the rate and yield of chemical reactions can be significantly increased with high catalytic efficiency.

Q&As

What other proteins does ATP5Ainteract with?

ATP5A1 interacts with other ATP synthase subunits and a variety of mitochondrial proteins (such as NADH dehydrogenase and chromosomal acyl-coa carboxylase) to participate in the process of energy metabolism.

Is the abnormal expression of ATP5Avaluable in the diagnosis and prognosis of neurodegenerative diseases?

The abnormal expression of ATP5A1 can be used as an important indicator for the diagnosis and prognosis of some neurodegenerative diseases, but it needs to be comprehensively analyzed in combination with other clinical and molecular biological results.

What is the mutation mechanism of ATP5A1?

Mutations in ATP5A1 may lead to abnormal changes in its intracellular localization and function, thereby affecting mitochondrial neuroregulation and energy metabolism.

What is the role of ATP5Ain biological development?

ATP5A1 participates in the regulation of cellular energy metabolism and mitochondrial function, and plays an important role in cell growth and development.

Are there any studies on drugs for ATP5Aregulating cellular energy metabolism?

Yes, several studies have been devoted to the development of drugs that can regulate the activity of ATP5A1 to affect cellular energy metabolism and treat related diseases.

Is the expression pattern of ATP5Aassociated with liver cancer or other tumors?

Yes, some studies have found that the expression pattern of ATP5A1 is related to the malignant degree and prognosis of liver cancer, bladder cancer, prostate cancer and other tumors.