Recombinant Human ATP5D, His-tagged

• Cat.No.: ATP5D-27063TH
• Product Overview: Recombinant full length Human ATP5D with N terminal His tag; 167 amino acids including tag, MWt 17.3 kDa.

Specification

• Description: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified.
• Protein length: 146 amino acids
• Conjugation: HIS
• Molecular Weight: 17.300kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, pH 8.0
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMAEAA AAPAAASGPNQMSFTFASPTQVFFN GANVRQVDVPTLTGAFGILAAHVPT LQVLRPGLVVVHAEDGTTSKYFVSS GSIAVNADSSVQLLAEEAVTLDMLD LGAAKANLEKAQAELVGTADEATRA EIQIRIEANEALVKALE
• Sequence Similarities: Belongs to the ATPase epsilon chain family.

Gene Information

• Gene Name: ATP5D ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit [ Homo sapiens ]
• Official Symbol: ATP5D
• Synonyms: ATP5D; ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit; ATP synthase subunit delta, mitochondrial
• Gene ID: 513
• mRNA Refseq: NM_001001975
• Protein Refseq: NP_001001975
• MIM: 603150
• Uniprot ID: P30049
• Chromosome Location: 19p13.3
• Pathway: Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Electron Transport Chain, organism-specific biosystem; F-type ATPase, eukaryotes, organism-specific biosystem; Formation of ATP by chemiosmotic coupling, organism-specific biosystem
• Function: contributes_to ADP binding; contributes_to ATP binding; contributes_to ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; protein complex binding

Reviews

08/01/2022

Because the remarkable purity of ATP5D makes it a valuable tool for sensitive and accurate experiments.

09/16/2020

The remarkable repeatability of ATP5D makes it an invaluable tool for accurate and reproducible experiments.

08/06/2019

The protein's purity and consistency make it a top choice for Western-blot experiments.

Q&As

In what diseases might ATP5D be a therapeutic target?

ATP5D may serve as a potential therapeutic target in some neurological and metabolic diseases.

Are ATP5D mutations associated with nervous system diseases?

Yes, mutations in the ATP5D gene are associated with certain neurological diseases such as multiple sclerosis, Parkinson's disease, etc.

What is the role of ATP5D protein in metabolic diseases?

ATP5D protein may be related to the occurrence and progression of metabolic diseases such as obesity and diabetes.

How does ATP5D function in tumors?

It may play a role in tumor cell metabolism and energy supply.

What is the biological function of ATP5D protein?

ATP5D is a subunit of the F1 portion of the mitochondrial ATP synthase complex involved in mitochondrial energy production.

What are the regulatory mechanisms of ATP5D?

The expression and function of ATP5D are affected by a variety of regulatory mechanisms, including regulation of transcription factors and epigenetic modifications.