Recombinant Human ATP5E 293 Cell Lysate

• Cat.No.: ATP5E-8602HCL

Specification

• Description: Antigen standard for ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit (ATP5E), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ATP5E ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit [ Homo sapiens ]
• Official Symbol: ATP5E
• Synonyms: ATP5E; ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit; ATP synthase subunit epsilon, mitochondrial; F(0)F(1)-ATPase; mitochondrial ATPase; H(+)-transporting two-sector ATPase; mitochondrial ATP synthase epsilon chain; ATPE; MC5DN3; MGC104243
• Gene ID: 514
• mRNA Refseq: NM_006886
• Protein Refseq: NP_008817
• MIM: 606153
• UniProt ID: P56381
• Chromosome Location: 20q13.3
• Pathway: Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Electron Transport Chain, organism-specific biosystem; F-type ATPase, eukaryotes, organism-specific biosystem; Formation of ATP by chemiosmotic coupling, organism-specific biosystem; Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem
• Function: contributes_to ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrolase activity; proton-transporting ATPase activity, rotational mechanism; transmembrane transporter activity

Reviews

09/26/2022

The manufacturer's products are derived from sustainable sources, ensuring minimal impact on the environment and local communities.

05/19/2022

This manufacturer's reputation for excellence in protein production is well deserved, with a focus on customer satisfaction and reliable delivery.

01/13/2019

Because proteins have short half-lives, they can be delivered more precisely and in a controlled manner, ensuring optimal therapeutic effects.

Q&As

What is the role of ATP5E in tumors?

ATP5E may play a role in tumor cell metabolism and energy supply.

Are ATP5E mutations associated with nervous system diseases?

Yes, ATP5E gene mutations are associated with certain neurological disorders such as Parkinson's disease, spinocerebellar ataxia, etc.

What is the role of ATP5E protein in metabolic diseases?

ATP5E protein may be associated with the occurrence and progression of metabolic diseases such as obesity and diabetes.

How are ATP5E mutations associated with disease?

Gene mutation may lead to abnormal protein structure or function, which in turn affects the physiological function of the protein and is associated with specific diseases.

What are the regulatory mechanisms of ATP5E?

ATP5E expression and function are affected by a variety of regulatory mechanisms, including regulation of transcription factors and epigenetic modifications.

In which tissues or organs is ATP5E highly expressed?

It is highly expressed in multiple tissues and organs, especially metabolically active tissues such as heart, liver, and brain.