|Product Overview:||Recombinant Human ATXN3L fused with His tag at C-terminal was expressed in Insect cells.|
|Description:||Ataxin-3-Like protein, also known as Machado-Joseph Disease (MJD) Protein-1-Like, is a 355 amino acid deubiquitinating (DUB) enzyme with a predicted molecular weight of 41 kDa. Full-length Ataxin-3-L contains an N-terminal Joesephin Domain, two Ubiquitin-Interacting-Motifs (UIM's) and a C-terminus consisting of a polyglutamine stretch and tail. Ataxin-3-L targets include Kruppel-Like Factor 5 (KLF5). KLF5 has been reported to promote breast cell proliferation, survival and tumorigenesis. Ubiquitination and proteasome-mediated degradation of KLF5 is antagonized by Ataxin-3-L, suggesting that inhibition of this DUB might provide a target for therapeutic intervention in breast cancer. Ataxin-3 and Ataxin-3-L have been reported to cleave both K48- and K63-linked poly-Ubiquitin in vitro, though enzyme activity in such assays is inefficient when using shorter chain lengths (≤ 6). Ataxin-3-L activity can be efficiently monitored with K63-linked tetra-Ubiquitin Rhodamine (UC-355) substrate.|
|Form:||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP|
|Bio-activity:||Recombinant Human His6-Ataxin-3-Like protein is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-Ataxin-3-like concentration of 10-100 nM when utilizing K63-linked tetra-Ubiquitin Rhodamine. Higher concentrations may be required for other substrates.|
|Molecular Mass:||Predicted Molecular Mass: 42 kDa|
|Purity:||>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
|Gene Name:||ATXN3L ataxin 3-like [ Homo sapiens ]|
|Synonyms:||ataxin 3-like; ATX3L; MJDL; EC 18.104.22.168; Machado-Joseph disease protein 1-like; EC 3.4.22; putative ataxin-3-like protein|
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