Active Recombinant Human ATXN3L protein, His-tagged

• Cat.No.: ATXN3L-203H
• Product Overview: Recombinant Human ATXN3L fused with His tag at C-terminal was expressed in Insect cells.

Specification

• Description: Ataxin-3-Like protein, also known as Machado-Joseph Disease (MJD) Protein-1-Like, is a 355 amino acid deubiquitinating (DUB) enzyme with a predicted molecular weight of 41 kDa. Full-length Ataxin-3-L contains an N-terminal Joesephin Domain, two Ubiquitin-Interacting-Motifs (UIM's) and a C-terminus consisting of a polyglutamine stretch and tail. Ataxin-3-L targets include Kruppel-Like Factor 5 (KLF5). KLF5 has been reported to promote breast cell proliferation, survival and tumorigenesis. Ubiquitination and proteasome-mediated degradation of KLF5 is antagonized by Ataxin-3-L, suggesting that inhibition of this DUB might provide a target for therapeutic intervention in breast cancer. Ataxin-3 and Ataxin-3-L have been reported to cleave both K48- and K63-linked poly-Ubiquitin in vitro, though enzyme activity in such assays is inefficient when using shorter chain lengths (≤ 6). Ataxin-3-L activity can be efficiently monitored with K63-linked tetra-Ubiquitin Rhodamine (UC-355) substrate.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
• Bio-activity: Recombinant Human His6-Ataxin-3-Like protein is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-Ataxin-3-like concentration of 10-100 nM when utilizing K63-linked tetra-Ubiquitin Rhodamine. Higher concentrations may be required for other substrates.
• Molecular Mass: Predicted Molecular Mass: 42 kDa
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: ATXN3L ataxin 3-like [ Homo sapiens ]
• Official Symbol: ATXN3L
• Synonyms: ataxin 3-like; ATX3L; MJDL; EC 3.4.19.12; Machado-Joseph disease protein 1-like; EC 3.4.22; putative ataxin-3-like protein
• Gene ID: 92552
• mRNA Refseq: NM_001135995.1
• Protein Refseq: NP_001129467.1
• UniProt ID: B4DYC7

Reviews

07/10/2021

Unmatched reproducibility of ATXN3L, with consistent results from experiment to experiment or application, makes it ideal for use in experiments or applications that require a high degree of reproducibility.

04/20/2021

This production process of ATXN3L is very environmentally friendly, without any harmful by-products and without any negative impact on the environment.

02/09/2019

The short half-life and high clearance properties of this recombinant protein make it a higher potential in drug development and is expected to become a new generation of therapeutic drugs.

Q&As

How does the ATXN3L protein interact with other proteins?

ATXN3L protein interacts with multiple proteins, including heat shock proteins, ubiquitin ligases and other regulatory proteins.

What is the regulatory mechanism of ATXN3L protein?

The expression and function of ATXN3L protein are affected by multiple regulatory mechanisms, including transcriptional regulation, post-translational modification, and protein-protein interaction.

What is the function of ATXN3L protein?

The protein plays a role in regulating protein degradation in cells, and participates in the process of cell quality control and waste protein degradation.

What diseases is ATXN3L involved in?

This protein is associated with nervous system related diseases, including Parkinson's disease, Alzheimer's disease and so on.

What are the structural features of ATXN3L protein?

The ATXN3L protein contains multiple functional domains, including multiple polyglutamic acid repeats, which may be involved in protein interactions and regulation of function.

How is ATXN3L protein expressed?

ATXN3L protein is expressed in various tissues of the human body, but its expression is higher in the central nervous system.