"ATXN3L" Related Products

Recombinant Human ATXN3L protein, His-tagged

Cat.No.: ATXN3L-203H
Product Overview: Recombinant Human ATXN3L fused with His tag at C-terminal was expressed in Insect cells.
Description: Ataxin-3-Like protein, also known as Machado-Joseph Disease (MJD) Protein-1-Like, is a 355 amino acid deubiquitinating (DUB) enzyme with a predicted molecular weight of 41 kDa. Full-length Ataxin-3-L contains an N-terminal Joesephin Domain, two Ubiquitin-Interacting-Motifs (UIM's) and a C-terminus consisting of a polyglutamine stretch and tail. Ataxin-3-L targets include Kruppel-Like Factor 5 (KLF5). KLF5 has been reported to promote breast cell proliferation, survival and tumorigenesis. Ubiquitination and proteasome-mediated degradation of KLF5 is antagonized by Ataxin-3-L, suggesting that inhibition of this DUB might provide a target for therapeutic intervention in breast cancer. Ataxin-3 and Ataxin-3-L have been reported to cleave both K48- and K63-linked poly-Ubiquitin in vitro, though enzyme activity in such assays is inefficient when using shorter chain lengths (≤ 6). Ataxin-3-L activity can be efficiently monitored with K63-linked tetra-Ubiquitin Rhodamine (UC-355) substrate.
Source: Insect cells
Species: Human
Tag : His
Form: X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Bio-activity: Recombinant Human His6-Ataxin-3-Like protein is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-Ataxin-3-like concentration of 10-100 nM when utilizing K63-linked tetra-Ubiquitin Rhodamine. Higher concentrations may be required for other substrates.
Molecular Mass: Predicted Molecular Mass: 42 kDa
Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
Gene Name: ATXN3L ataxin 3-like [ Homo sapiens ]
Official Symbol: ATXN3L
Synonyms: ataxin 3-like; ATX3L; MJDL; EC 3.4.19.12; Machado-Joseph disease protein 1-like; EC 3.4.22; putative ataxin-3-like protein
Gene ID: 92552
mRNA Refseq: NM_001135995.1
Protein Refseq: NP_001129467.1
UniProt ID: B4DYC7

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.