Recombinant Human AURKB Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: AURKB-5410H
• Product Overview: AURKB MS Standard C13 and N15-labeled recombinant protein (NP_004208) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 39.1 kDa
• AA Sequence: MAQKENSYPWPYGRQTAPSGLSTLP QRVLRKEPVTPSALVLMSRSNVQPT AAPGQKVMENSSGTPDILTRHFTID DFEIGRPLGKGKFGNVYLAREKKSH FIVALKVLFKSQIEKEGVEHQLRRE IEIQAHLHHPNILRLYNYFYDRRRI YLILEYAPRGELYKELQKSCTFDEQ RTATIMEELADALMYCHGKKVIHRD IKPENLLLGLKGELKIADFGWSVHA PSLRRKTMCGTLDYLPPEMIEGRMH NEKVDLWCIGVLCYELLVGNPPFES ASHNETYRRIVKVDLKFPASVPTGA QDLISKLLRHNPSERLPLAQVSAHP WVRANSRRVLPPSALQSVATRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: AURKB aurora kinase B [ Homo sapiens (human) ]
• Official Symbol: AURKB
• Synonyms: AURKB; aurora kinase B; serine/threonine kinase 12, STK12; Aik2; AIM 1; ARK2; AurB; aurora 1; aurora B; IPL1; PPP1R48; protein phosphatase 1; regulatory subunit 48; STK5; ARK-2; STK-1; aurora-1; aurora-B; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora-related kinase 2; serine/threonine kinase 12; aurora/IPL1-related kinase 2; serine/threonine-protein kinase 5; serine/threonine-protein kinase 12; serine/threonine-protein kinase aurora-B; protein phosphatase 1, regulatory subunit 48; aurora- and Ipl1-like midbody-associated protein 1; AIK2; AIM1; AIM-1; STK12; aurkb-sv1; aurkb-sv2
• Gene ID: 9212
• mRNA Refseq: NM_004217
• Protein Refseq: NP_004208
• MIM: 604970
• UniProt ID: Q96GD4

Reviews

12/28/2021

AURKB's exceptional repeatability allows for its reliable use in a range of applications, making it a top choice for researchers.

08/31/2021

Very pure and can be used with confidence.

02/03/2019

The quality is very high and very reliable.

Q&As

What are the interactions between AURKB protein and cell cycle regulatory proteins?

AURKB protein interacts with a variety of cell cycle regulatory proteins, including Cdk1, INCENP, Survivin, etc., to regulate the progression of cell mitosis.

What are the intracellular substrates of AURKB protein?

The substrates of AURKB include chromosome-associated proteins, cytoskeletal proteins and other cell cycle regulatory proteins. Phosphorylation modification of these substrates regulates chromosome segregation and cell division during mitosis.

What is the cellular localization of AURKB protein?

AURKB protein is mainly located in the nucleus and cytoplasm and participates in the regulation of cell mitosis.

What is the expression pattern of AURKB gene?

The expression of the AURKB gene is regulated by cell cycle regulators and other transcription factors and is mainly expressed in the mitotic phase of cells.

How does AURKB protein activity work?

The activity of AURKB protein is regulated by a variety of factors, including phosphorylation modification, protein interaction and cell cycle regulation.

At what stage of cell mitosis does AURKB protein reach its peak expression?

The protein reaches its peak expression during early and metaphase of cell mitosis and is closely associated with chromosome segregation and cell division.