Recombinant Human Aurora Kinase A, His-tagged

• Cat.No.: AURKA-683H
• Product Overview: Recombinant human Aurora A kinase (amino acids 2-403) fused at the N-terminus to a His Tag sequence was expressed inS. frugiperdainsect cells using a baculovirus expression system. Aurora A plays an important role in chromosome segregation and cell division. MW = 50 kDa.

Specification

• Cat. No.: AURKA-683H
• Description: Aurora A kinase also known as serine/threonine-protein kinase 6 is an enzyme that in humans is encoded by the AURKA gene. Aurora A is a member of a family of mitotic serine/threonine kinases. It is implicated with important processes during mitosis and meiosis whose proper function is integral for healthy cell proliferation. Aurora A is activated by one or more phosphorylations and its activity peaks during the G2 phase to M phase transition in the cell cycle.
• Source: S. frugiperda.
• Form: Liquid. In 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween-20 Detergent, pH 7.5. Please refer to vial label for lot-specific concentration.
• Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per min at 30°C, pH 7.4.
• Purity: ≥90% by SDS-PAGE.
• Specific Activity: ≥120 U/µg protein.
• Toxicity: Regulatory Review.
• Storage: ≤ -70°C. Avoid freeze/thaw Following initial thaw, aliquot and freeze (-70°C).

Gene Information

• Gene Name: AURKA aurora kinase A [ Homo sapiens ]
• Synonyms:
  aurora kinase A; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; MGC34538; AURKA; serine/threonine-protein kinase 6; ARK-1; hARK1; OTTHUMP00000031341; OTTHUMP00000166072; IPL1-related kinase; aurora-related kinase 1; aurora/IPL1-like kinase; serine/threonine kinase 6; aurora/IPL1-related kinase 1; breast tumor-amplified kinase; breast-tumor-amplified kinase; serine/threonine protein kinase 15; serine/threonine-protein kinase 15; serine/threonine-protein kinase aurora-A; Aurora/IPL1-related kinase 1; Serine/threonine-protein kinase aurora-A; Aurora-related kinase 1; Serine/threonine-protein kinase 15; Breast tumor-amplified kinase; EC 2.7.11.1; AurA
• Gene ID: 6790
• mRNA Refseq: NM_003600
• Protein Refseq: NP_003591
• MIM: 603072
• UniProt ID: O14965
• Chromosome Location: 20q13.2-q13.3
• Pathway: Oocyte meiosis; Cell Cycle, Mitotic
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; transferase activity; ubiquitin protein ligase binding

Reviews

12/08/2022

AURKA's exceptional stability allows for its reliable use in a range of applications, making it a top choice for researchers.

02/10/2022

This protein's exceptional activity allows for its reliable use in a range of applications, making it a top choice for researchers.

10/24/2020

Due to the extensive storage conditions of proteins, the experimental process is more stable and reliable, which provides a strong guarantee for scientific research.

Q&As

How does AURKA interact with cell cycle regulators?

AURKA protein interacts with a variety of cell cycle regulatory proteins, including Cdk1, Plk1, TPX2, etc., to regulate the progression of cell cycle.

What is the role of AURKA protein in the cell cycle?

The protein plays an important role in the G2 and M phases of the cell cycle and is involved in the regulation of nuclear division, chromosome segregation, and cell mitosis.

Is AURKA protein involved in apoptosis and DNA damage repair?

AURKA protein may be involved in the regulation of apoptosis and DNA damage repair, but the specific mechanism needs to be further studied.

What is the regulatory mechanism of AURKA protein activity?

The activity is regulated by a variety of factors, including phosphorylation modification, protein interaction and cell cycle regulation.

Where are the cells of AURKA protein?

It is mainly localized in the cytoplasm and nucleus and is involved in cell cycle regulation and cell division processes.

What is the role of AURKA protein in tumorigenesis?

This protein plays an important role in tumorigenesis, and its overexpression or mutation may lead to abnormal cell proliferation, chromosome instability and tumorigenesis.