Recombinant Human Aurora Kinase B, GST-tagged, Active

• Cat.No.: AURKB-247H
• Product Overview: Recombinant full-length human AURORAB was expressed by baculovirus inSf9 insect cellsusing a N-terminal GST tag. MW = 68 kDa.

Specification

• Cat. No.: AURKB-247H
• Description: AURORA B is a member of the Aurora kinase family that associates with microtubules during chromosome movement and segregation. AURORA B localizes to the microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. AURORA B inhibits the microtubule depolymerizing activity of mitotic centromere-associated kinesin (MCAK) by phosphorylating MCAK on Ser92. This phosphorylation also regulates MCAK translocalization from kinetochores to the centromere. AURORA B has been identified as a target for the development of new anticancer agents since inhibition of AURORA B gives rise to the more pronounced antiproliferative phenotype.
• Source: Sf9 insect cells using baculovirus.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: AURKB aurora kinase B [ Homo sapiens ]
• Synonyms: AURKB; aurora kinase B ; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; aurkb-sv1; aurkb-sv2; aurora-1; aurora-B; aurora kinase B-Sv1; aurora kinase B-Sv2; serine/threonine kinase 12; EC 2.7.11.1; Aurora- and Ipl1-like midbody-associated protein 1; Aurora-related kinase 2; Aurora/IPL1-related kinase 2
• Gene ID: 9212
• mRNA Refseq: NM_004217
• Protein Refseq: NP_004208
• MIM: 604970
• UniProt ID: Q96GD4
• Chromosome Location: 17p13.1
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity

Reviews

12/28/2021

AURKB's exceptional repeatability allows for its reliable use in a range of applications, making it a top choice for researchers.

08/31/2021

Very pure and can be used with confidence.

02/03/2019

The quality is very high and very reliable.

Q&As

What are the interactions between AURKB protein and cell cycle regulatory proteins?

AURKB protein interacts with a variety of cell cycle regulatory proteins, including Cdk1, INCENP, Survivin, etc., to regulate the progression of cell mitosis.

What are the intracellular substrates of AURKB protein?

The substrates of AURKB include chromosome-associated proteins, cytoskeletal proteins and other cell cycle regulatory proteins. Phosphorylation modification of these substrates regulates chromosome segregation and cell division during mitosis.

What is the cellular localization of AURKB protein?

AURKB protein is mainly located in the nucleus and cytoplasm and participates in the regulation of cell mitosis.

What is the expression pattern of AURKB gene?

The expression of the AURKB gene is regulated by cell cycle regulators and other transcription factors and is mainly expressed in the mitotic phase of cells.

How does AURKB protein activity work?

The activity of AURKB protein is regulated by a variety of factors, including phosphorylation modification, protein interaction and cell cycle regulation.

At what stage of cell mitosis does AURKB protein reach its peak expression?

The protein reaches its peak expression during early and metaphase of cell mitosis and is closely associated with chromosome segregation and cell division.