Recombinant Human BAAT Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: BAAT-1425H
• Product Overview: BAAT MS Standard C13 and N15-labeled recombinant protein (NP_001121082) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.3 kDa
• AA Sequence: MIQLTATPVSALVDEPVHIQATGLI PFQMVSFQASLEDENGDMFYSQAHY RANEFGEVDLNHASSLGGDYMGVHP MGLFWSLKPEKLLTRLLKRDVMNRP FQVQVKLYDLELIVNNKVASAPKAS LTLERWYVAPGVTRIKVREGRLRGA LFLPPGEGLFPGVIDLFGGLGGLLE FRASLLASRGFASLALAYHNYEDLP RKPEVTDLEYFEEAANFLLRHPKVF GSGVGVVSVCQGVQIGLSMAIYLKQ VTATVLINGTNFPFGIPQVYHGQIH QPLPHSAQLISTNALGLLELYRTFE TTQVGASQYLFPIEEAQGQFLFIVG EGDKTINSKAHAEQAIGQLKRHGKN NWTLLSYPGAGHLIEPPYSPLCCAS TTHDLRLHWGGEVIPHAAAQEHAWK EIQRFLRKHLIPDVTSQLTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: BAAT bile acid-CoA:amino acid N-acyltransferase [ Homo sapiens (human) ]
• Official Symbol: BAAT
• Synonyms: BAAT; bile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase); bile acid Coenzyme A: amino acid N acyltransferase (glycine N choloyltransferase); bile acid-CoA:amino acid N-acyltransferase; BAT; long-chain fatty-acyl-CoA hydrolase; bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase); BACAT; FLJ20300; MGC104432
• Gene ID: 570
• mRNA Refseq: NM_001127610
• Protein Refseq: NP_001121082
• MIM: 602938
• UniProt ID: Q14032