Recombinant Human Brain Natriuretic Peptide (type B natriuretic peptide), His-tagged

• Cat.No.: NPPB-369H
• Product Overview: Recombinant human BNP, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 14 kDa (129 aa).

Specification

• Cat. No.: NPPB-369H
• Description: BNP (Brain natriuretic peptide) is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. BNP is a 32 amino acid polypeptide and is co-secreted along with a 76 amino acid N-terminal fragment which is biologically inactive. This protein has biological actions including natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MHPLGSPGSA SDLETSGLQE QRNHLQGKLS ELQVEQTSLE PLQESPRPTG VWKSREVATE GIRGHRKMVL YTLRAPRSPK MVQGSGCFGR KMDRISSSSG LGCKVLRRH
• Formulation: Liquid in Phosphate-Buffered Saline (pH 7.4), 40%glycerol, 2mM DTT, 0.1mM PMSF, 1mM EDTA
• Molecular Weight: 14 kDa (129 aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Endotoxin Level: < 1.0 eu per 1 μg of protein (determined by lal>
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: NPPB natriuretic peptide precursor B [ Homo sapiens ]
• Synonyms: BNP; OTTHUMP00000002506; OTTHUMP00000044486; Gamma-brain natriuretic peptide; brain type natriuretic peptide; natriuretic peptide precursor B; natriuretic protein; NPPB; Natriuretic peptides B; Gamma-brain natriuretic peptide; GC-B
• Gene ID: 4879
• mRNA Refseq: NM_002521
• Protein Refseq: NP_002512
• MIM: 600295
• UniProt ID: P16860
• Chromosome Location: 1p36.2
• Function: diuretic hormone activity

Reviews

06/17/2022

They actively engage with researchers, comprehending their specific experimental needs, and offering tailored services accordingly.

01/16/2022

This collaborative partnership facilitates a seamless and efficient research process, as the manufacturer aligns their support and services with my unique requirements.

12/09/2019

The manufacturer's excellent technical support, commitment to innovation, and customer-centric approach further reinforce its suitability for my research.

Q&As

In what ways does NPPB protein contribute to the understanding of fluid overload in patients?

Elevated NPPB levels are associated with fluid overload, providing valuable information for clinicians in managing patients with conditions like edema.

Can NPPB protein be used in monitoring the efficacy of treatments for cardiovascular diseases?

Yes, monitoring NPPB levels over time can help assess the effectiveness of treatments and interventions in managing cardiovascular conditions.

How do age and gender influence the normal range of NPPB protein levels in healthy individuals?

Normal ranges of NPPB may vary with age and gender. Understanding these variations is essential for accurate clinical interpretation.

Are there any specific contraindications or limitations in using NPPB protein as a clinical marker?

While NPPB is a valuable marker, clinicians should consider factors like renal function and comorbidities that may affect its interpretation.

How does NPPB protein influence the management of hypertension in clinical practice?

NPPB regulates blood pressure by promoting vasodilation and natriuresis. Understanding its levels can guide the management of hypertension.