Recombinant Human CAMK2B protein(Met1-Gln503), His&GST-tagged

• Cat.No.: CAMK2B-970H
• Product Overview: Recombinant Human CAMK2B isoform 2 (Q13554-3) (Met 1-Gln 503) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His&GST
• Protein length: Met1-Gln503
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 2mM GSH, 0.5mM PMSF, 10% gly, pH 8.0
• Bio-activity: The specific activity was determined to be 300 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.
• Molecular Mass: The recombinant human CAMK2B/GST chimera consists of 740 amino acids and has a calculated molecular mass of 84.2 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 60 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CAMK2B calcium/calmodulin-dependent protein kinase II beta [ Homo sapiens ]
• Official Symbol: CAMK2B
• Synonyms: CAMK2B; calcium/calmodulin-dependent protein kinase II beta; calcium/calmodulin dependent protein kinase (CaM kinase) II beta , CAMKB; calcium/calmodulin-dependent protein kinase type II subunit beta; calcium/calmodulin dependent protein kinase type II beta chain; CaM kinase II beta subunit; CaM kinase II beta chain; CAM2; CAMK2; proline rich calmodulin dependent protein kinase; caMK-II subunit beta; CaM-kinase II beta chain; proline rich calmodulin-dependent protein kinase; CAMKB; MGC29528
• Gene ID: 816
• mRNA Refseq: NM_001220
• Protein Refseq: NP_001211
• MIM: 607707
• UniProt ID: Q13554