| Description : |
Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. |
| Source : |
Wheat Germ |
| Species : |
Human |
| Tag : |
GST |
| Form : |
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
| Molecular Mass : |
101.3 kDa |
| Protein length : |
1 a.a. - 672 a.a. |
| AA Sequence : |
MRAGRGATPARELFRDAAFPAADSS LFCDLSTPLAQFREDITWRRPQEIC ATPRLFPDDPREGQVKQGLLGDCWF LCACAALQKSRHLLDQVIPPGQPSW ADQEYRGSFTCRIWQFGRWVEVTTD DRLPCLAGRLCFSRCQREDVFWLPL LEKVYAKVHGSYEHLWAGQVADALV DLTGGLAERWNLKGVAGSGGQQDRP GRWEHRTCRQLLHLKDQCLISCCVL SPRAGARELGEFHAFIVSDLRELQG QAGQCILLLRIQNPWGRRCWQGLWR EGGEGWSQVDAAVASELLSQLQEGE FWVEEEEFLREFDELTVGYPVTEAG HLQSLYTERLLCHTRALPGAWVKGQ SAGGCRNNSGFPSNPKFWLRVSEPS EVYIAVLQRSRLHAADWAGRARALV GDSHTSWSPASIPGKHYQAVGLHLW KVEKRRVNLPRVLSMPPVAGTACHA YDREVHLRCELSPGYYLAVPSTFLK DAPGEFLLRVFSTGRVSLSAIRAVA KNTTPGAALPAGEWGTVQLRGSWRV GQTAGGSRNFASYPTNPCFPFSVPE GPGPRCVRITLHQHCRPSDTEFHPI GFHIFQVPEGGRSQDAPPLLLQEPL LSCVPHRYAQEVSRLCLLPAGTYKV VPSTYLPDTEGAFTVTIATRIDRPS IHSQEMLGQFLQEVSVMAVMKT |
| Applications : |
Enzyme-linked Immunoabsorbent; Assay Western Blot (Recombinant protein); Antibody Production; Protein Array |
| Notes : |
Best use within three months from the date of receipt of this protein. |
| Storage : |
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
