Recombinant Human CAST Protein, His&GST-tagged
Cat.No. : | CAST-628H |
Product Overview : | Recombinant Human CAST Protein (Ala446-Lys636) with His&GST tag was expressed in E. coli. |
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Description : | The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. |
Source : | E. coli |
Species : | Human |
Tag : | N-His&GST |
Form : | Freeze-dried powder |
Molecular Mass : | Predicted Molecular Mass: 51.3 kDa; Accurate Molecular Mass: 58 kDa |
Protein length : | Ala446-Lys636 |
Purity : | > 80% |
Applications : | Positive Control; Immunogen; SDS-PAGE; WB. |
Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months. |
Storage Buffer : | 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name : | CAST calpastatin [ Homo sapiens (human) ] |
Official Symbol : | CAST |
Synonyms : | CAST; calpastatin; calpain inhibitor; sperm BS-17 component; BS-17; MGC9402; |
Gene ID : | 831 |
mRNA Refseq : | NM_001042440 |
Protein Refseq : | NP_001035905 |
MIM : | 114090 |
UniProt ID : | P20810 |
Products Types
◆ Recombinant Protein | ||
CAST-0441H | Recombinant Human CAST Protein, GST-Tagged | +Inquiry |
Cast-776M | Recombinant Mouse Cast Protein, MYC/DDK-tagged | +Inquiry |
CAST-1350H | Recombinant Human CAST Protein (1-667 aa), His-tagged | +Inquiry |
CAST-807R | Recombinant Rat CAST Protein, His (Fc)-Avi-tagged | +Inquiry |
CAST-1434H | Recombinant Human CAST Protein (Ala446-Lys636), N-His tagged | +Inquiry |
◆ Lysates | ||
CAST-7824HCL | Recombinant Human CAST 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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