| Description : |
Chemokine (C-C motif) ligand 22(ABCD-1 / CCL22)is a kind of CC chemokine which is a family of secreted proteins involved in immunoregulatory and inflammatory pr centigradeesses. The cytokine displays chemotactic activity for mon centigradeytes, dendritic cells, natural killer cells and for chronically activated T lymph centigradeytes. It also displays a mild activity for primary activated T lymph centigradeytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymph centigradeytes. This ABCD-1 / CCL22 chemokine binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated / effector T lymph centigradeytes to inflammatory sites and other aspects of activated T-lymph centigradeyte physiology. ABCD-1 / CCL22 is highly expressed in macrophage and in mon centigradeyte-derived dendritic cells, and thymus, and in Langerhans' cell histi centigradeytosis and atopic dermatitis but not in dermatopathic lymphadenopathy. This chemokine is also found in lymph node, appendix, activated mon centigradeytes, resting and activated macrophages. This protein is lower expressed in lung and spleen and very weekly expressed in small intestine. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Predicted N Terminal : |
Met |
| Form : |
Lyophilized from sterile 30% Acetonitrile, 0.1% TFA. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Molecular Mass : |
The recombinant human CCL22 consisting of 84 amino acids and has a calculated molecular mass of 9.7 KDa. It migrates as an 10 kDa band in SDS-PAGE under reducing conditions. |
| Protein length : |
Gly 25-Gln 93 |
| Endotoxin : |
< 1.0 EU per μg protein as determined by the LAL method. |
| Purity : |
> 90 % as determined by SDS-PAGE. |
| Storage : |
Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
