Active Recombinant Human CD276 protein, His-Avi-tagged, Biotinylated

• Cat.No.: CD276-1286H
• Product Overview: Biotinylated Recombinant Human CD276 protein(Leu 29 - Pro 245), fused with His-Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H3, Avitag,His Tag with a linear range of 5-39 ng/mL.Immobilized Biotinylated Human B7-H3, Avitag,His Tag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL.
• Molecular Mass: This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.The protein has a calculated MW of 26.5 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Leu 29 - Pro 245
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD276 CD276 molecule [ Homo sapiens ]
• Official Symbol: CD276
• Synonyms: CD276; CD276 molecule; CD276 antigen; B7 H3; B7H3; B7RP 2; B7 homolog 3; costimulatory molecule; B7-H3; B7RP-2; 4Ig-B7-H3
• Gene ID: 80381
• mRNA Refseq: NM_001024736
• Protein Refseq: NP_001019907
• MIM: 605715
• UniProt ID: Q5ZPR3

Reviews

03/15/2022

It has consistently demonstrated high sensitivity and accuracy in detecting and quantifying target molecules, allowing for precise and reliable measurement of analytes in my experimental samples.

08/06/2021

Its versatility and reliability make it an excellent choice for research applications, and I highly recommend it to fellow scientists.

06/03/2021

The sensitivity and specificity of the CD276 Protein have consistently delivered robust and consistent results, enhancing the efficiency and accuracy of my experimental workflows.

Q&As

In which other diseases, besides cancer, is CD276 implicated?

CD276 has been associated with autoimmune diseases and certain inflammatory conditions. Exploring its role in these diseases may open avenues for therapeutic interventions.

Are there diagnostic applications for CD276 in cancer detection?

Research suggests that CD276 expression levels may serve as a diagnostic biomarker for certain cancers. Detection methods, such as immunohistochemistry, are being explored to assess CD276 levels in tumor tissues.

How does CD276 influence the tumor microenvironment?

CD276 can modulate the tumor microenvironment by suppressing immune responses, promoting angiogenesis, and supporting tumor cell survival. Understanding these interactions is crucial for developing targeted therapies.

Are there any ongoing clinical trials focusing on CD276 in cancer patients?

Yes, several clinical trials are underway to evaluate the safety and efficacy of CD276-targeted therapies in different cancer types. These trials aim to assess the impact on patient outcomes and potential side effects.

How does CD276-targeted therapy differ from traditional cancer treatments?

CD276-targeted therapy focuses on modulating the immune response against cancer cells, whereas traditional treatments like chemotherapy and radiation target rapidly dividing cells, including both healthy and cancerous cells.