Recombinant Human CD44 Protein, hFc-tagged, Alexa Fluor 488 conjugated
Cat.No. : | CD44-3095HAF488 |
Product Overview : | Alexa Fluor 488 conjugated recombinant human CD44 (Gln21-Pro220) protein (HPLC-verified) was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293). |
Unit | |
Price | |
Qty |
- Specification
- Gene Information
- Related Products
- Download
Description : | CD44 antigen is a cell-surface glycoprotein involved in cell–cell interactions, cell adhesion and migration. CD44 is expressed in a large number of mammalian cell types. CD44 is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). CD44 function is controlled by its posttranslational modifications. One critical modification involves discrete sialofucosylations rendering the selectin-binding glycoform of CD44 called HCELL (for Hematopoietic Cell E-selectin/L-selectin Ligand). CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms. |
Source : | HEK293 |
Species : | Human |
Tag : | hFc |
Form : | Lyophilized |
Molecular Mass : | This protein has a calculated MW of 48.7 kDa. The reducing (R) protein migrates as 76 kDa in SDS-PAGE due to glycosylation. |
Protein length : | Gln21-Pro220 |
N-terminal Sequence Analysis : | Gln 21 |
Endotoxin : | < 1.0 EU/ μg by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE |
Characteristic : | Disulfide-linked homodimer Labeled with Alexa Fluor 488 via amines Excitation Wavelength: 488 nm Emission Wavelength: 515-545 nm |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage Buffer : | Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | CD44 |
Official Symbol : | CD44 |
Synonyms : | CD44; CD44 molecule (Indian blood group); CD44 antigen (homing function and Indian blood group system) , MDU2, MDU3, MIC4; CD44 antigen; CD44R; chondroitin sulfate proteoglycan 8; CSPG8; HCELL; hematopoietic cell E and L selectin ligand; IN; MC56; Pgp1; epican; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; heparan sulfate proteoglycan; cell surface glycoprotein CD44; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; homing function and Indian blood group system; LHR; MDU2; MDU3; MIC4; CDW44; HUTCH-I; ECMR-III; MGC10468 |
Gene ID : | 960 |
mRNA Refseq : | NM_000610 |
Protein Refseq : | NP_000601 |
MIM : | 107269 |
UniProt ID : | P16070 |
Products Types
◆ Recombinant Protein | ||
CD44-684H | Recombinant Human CD44 Protein, His-tagged | +Inquiry |
CD44-0821H | Recombinant Human CD44 Protein, GST-tagged | +Inquiry |
CD44-160H | Recombinant Human CD44 Protein, His and Strep-tagged | +Inquiry |
CD44-0820H | Recombinant Human CD44 Protein | +Inquiry |
CD44-001H | Active Recombinant Human CD44 Protein, Myc/DDK-tagged | +Inquiry |
◆ Lysates | ||
CD44-1090HCL | Recombinant Human CD44 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (7)
Ask a questionCD44 is implicated in cancer progression and metastasis. It promotes tumor cell invasion, migration, and adhesion to the extracellular matrix. CD44 also plays a role in cancer stem cell maintenance and drug resistance. Its overexpression is associated with poor prognosis in various types of cancer.
Targeting CD44 has been explored as a potential therapeutic strategy in cancer and inflammatory diseases. Strategies include blocking CD44-ligand interactions, inhibiting CD44 signaling pathways, and targeting CD44-positive cancer stem cells. However, further research is needed to fully understand the complex roles of CD44 in different disease contexts and to develop effective CD44-targeted therapies.
The expression of CD44 is regulated at multiple levels, including transcriptional control, alternative splicing, and post-translational modifications. Transcription factors such as Sp1 and NF-κB can bind to the CD44 promoter region to regulate its gene expression. Additionally, alternative splicing of CD44 pre-mRNA generates CD44 isoforms with different functions and expression patterns.
CD44 is involved in immune cell activation, trafficking, and homing. It participates in the recruitment of leukocytes to sites of inflammation and mediates T cell adhesion and migration. CD44 can also modulate immune responses by interacting with other immune receptors and cytokines, and it is involved in the regulation of T cell activation and differentiation.
CD44 plays a vital role in immune regulation by participating in immune cell activation, migration, and modulation of T-cell activation and differentiation. CD44 is expressed on various immune cell populations, including T cells, B cells, natural killer cells, and dendritic cells. It serves as an adhesion molecule and facilitates immune cell trafficking to lymphoid tissues and inflammatory sites. CD44 also functions as a co-stimulatory receptor, enhancing T-cell activation and cytokine production upon interaction with its ligands. Moreover, CD44 isoforms have been implicated in the differentiation of T helper cell subsets, such as Th1, Th2, and Th17 cells. These findings highlight the multifaceted role of CD44 in immune regulation and its potential as a therapeutic target for immune-related disorders.
CD44 is a transmembrane glycoprotein predominantly localized on the cell surface, specifically in the plasma membrane of various cell types, including epithelial cells, leukocytes, and mesenchymal cells.
CD44 can activate various signaling pathways, including PI3K/AKT, MAPK/ERK, and NF-κB pathways. These pathways mediate downstream cellular processes such as cell survival, proliferation, migration, and immune responses. CD44 can also interact with other cell surface receptors, such as receptor tyrosine kinases, to modulate their signaling activities.
Customer Reviews (3)
Write a reviewUsing this reagent, I can gain a better understanding of the functionality and regulatory mechanisms of proteins.
This reagent has long-lasting performance, enduring prolonged experimental use.
A miraculous reagent driving us towards the frontiers, inviting a glance into an expanded world.
Ask a Question for All CD44 Products
Required fields are marked with *
My Review for All CD44 Products
Required fields are marked with *
Inquiry Basket