Recombinant Human CDH2 protein(Met1-Ala724), His-tagged

• Cat.No.: CDH2-2191H
• Product Overview: Recombinant Human CDH2 (NP_001783.2) (Met 1-Ala 724) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Ala724
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When 5 x 10^4 cells/well are added to CDH2 coated plates (10 μg/mL with 100 μL/well), approximately 60% will adhere after 1 hour at 37°C.
• Molecular Mass: The pro form of human CDH2 consists of 710 amino acids and predictes a molecular mass of 78.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of CDH2 is 90 & 75 kDa corresponding to the pro fom and mature form respectinely due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CDH2 cadherin 2, type 1, N-cadherin (neuronal) [ Homo sapiens ]
• Official Symbol: CDH2
• Synonyms: CDH2; cadherin 2, type 1, N-cadherin (neuronal); NCAD; cadherin-2; CD325; CDHN; N cadherin; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal; CDw325
• Gene ID: 1000
• mRNA Refseq: NM_001792
• Protein Refseq: NP_001783
• MIM: 114020
• UniProt ID: P19022