Recombinant Human CENPF Protein (1759-2093 aa), His-MBP-tagged

• Cat.No.: CENPF-2743H
• Product Overview: Recombinant Human CENPF Protein (1759-2093 aa) is produced by Baculovirus expression system. This protein is fused with a MBP tag at the N-terminal and a 6xHis tag at the C-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Partial.

Specification

• Description: Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.
• Source: Baculovirus
• Species: Human
• Tag: His-MBP
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 82.2 kDa
• Protein length: 1759-2093 aa
• AA Sequence: AIQGRNESCDISKEHTSETTERTPK HDVHQICDKDAQQDLNLDIEKITET GAVKPTGECSGEQSPDTNYEPPGED KTQGSSECISELSFSGPNALVPMDF LGNQEDIHNLQLRVKETSNENLRLL HVIEDRDRKVESLLNEMKELDSKLH LQEVQLMTKIEACIELEKIVGELKK ENSDLSEKLEYFSCDHQELLQRVET SEGLNSDLEMHADKSSREDIGDNVA KVNDSWKERFLDVENELSRIRSEKA SIEHEALYLEADLEVVQTEKLCLEK DNENKQKVIVCLEEELSVVTSERNQ LRGELDTMSKKTTALDQLSEKMKEK TQELESHQSE
• Purity: > 85% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: CENPF centromere protein F, 350/400kDa (mitosin) [ Homo sapiens ]
• Official Symbol: CENPF
• Synonyms: CENPF; centromere protein F; hcp 1; mitosin; AH antigen; kinetochore protein CENPF; CENF; hcp-1; PRO1779
• Gene ID: 1063
• mRNA Refseq: NM_016343
• Protein Refseq: NP_057427
• MIM: 600236
• UniProt ID: P49454