Recombinant Human CENPH Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CENPH-745H
• Product Overview: CENPH MS Standard C13 and N15-labeled recombinant protein (NP_075060) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 28.5 kDa
• AA Sequence: MEEQPQMQDADEPADSGGEGRAGGP PQVAGAQAACSEDRMTLLLRLRAQT KQQLLEYKSMVDASEEKTPEQIMQE KQIEAKIEDLENEIEEVKVAFEIKK LALDRMRLSTALKKNLEKISRQSSV LMDNMKHLLELNKLIMKSQQESWDL EEKLLDIRKKRLQLKQASESKLLEI QTEKNKQKIDLDSMENSERIKIIRQ NLQMEIKITTVIQHVFQNLILGSKV NWAEDPALKEIVLQLEKNVDMMTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CENPH centromere protein H [ Homo sapiens (human) ]
• Official Symbol: CENPH
• Synonyms: CENPH; centromere protein H; NNF1; MIND kinetochore complex component; homolog (S. cerevisiae); PMF1; CENP-H; kinetochore protein CENP-H; interphase centromere complex protein 35
• Gene ID: 64946
• mRNA Refseq: NM_022909
• Protein Refseq: NP_075060
• MIM: 605607
• UniProt ID: Q9H3R5