Recombinant Human CHI3L1 Protein, GST-Tagged

• Cat.No.: CHI3L1-1232H
• Product Overview: Human CHI3L1 full-length ORF (AAH08568.1, 1 a.a. - 383 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 69 kDa
• AA Sequence: MGVKASQTGFVVLVLLQCCSAYKLV CYYTSWSQYREGDGSCFPDALDRFL CTHIIYSFANISNDHIDTWEWNDVT LYGMLNTLKNRNPNLKTLLSVGGWN FGSQRFSKIASNTQSRRTFIKSVPP FLRTHGFDGLDLAWLYPGRRDKQHF TTLIKEMKAEFIKEAQPGKKQLLLS AALSAGKVTIDSSYDIAKISQHLDF ISIMTYDFHGAWRGTTGHHSPLFRG QEDASPDRFSNTDYAVGYMLRLGAP ASKLVMGIPTFGRSFTLASSETGVG APISGPGIPGRFTKEAGTLAYYEIC DFLRGATVHRILGQQVPYATKGNQW VGYDDQESVKSKVQYLKDRQLAGAM VWALDLDDFQGSFCGQDLRFPLTNA IKDALAAT
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CHI3L1 chitinase 3-like 1 (cartilage glycoprotein-39) [ Homo sapiens ]
• Official Symbol: CHI3L1
• Synonyms: CHI3L1; chitinase 3-like 1 (cartilage glycoprotein-39); chitinase-3-like protein 1; GP39; YKL40; 39 kDa synovial protein; cartilage glycoprotein 39; ASRT7; GP-39; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39; FLJ38139; DKFZp686N19119
• Gene ID: 1116
• mRNA Refseq: NM_001276
• Protein Refseq: NP_001267
• MIM: 601525
• UniProt ID: P36222

Reviews

12/15/2022

CHI3L1 has strong biological activity combined with its high purity makes its experimental results extremely reliable.

10/22/2022

The band strength in Western blot was high, which improved the signal-to-noise ratio of the experiment.

10/24/2021

It can simplify the complex chemical reaction and improve the high yield, shorten the experimental time.

Q&As

Is there any other mode of action for CHI3L1?

In addition to being involved in cartilage metabolism and immune regulation, CHI3L1 may also have other modes of action, such as regulating cell adhesion and migration.

What is the role of CHI3L1 in immune regulation?

This protein can act as an immunomodulator to inhibit the inflammatory response and the activity of immune cells, thereby maintaining the balance of the immune system.

Does CHI3L1 involved in the metabolism and repair of cartilage?

CHI3L1 can bind to receptors on the surface of chondrocytes, stimulate the proliferation and differentiation of chondrocytes, and promote cartilage synthesis and repair.

How is the expression level of CHI3L1 regulated?

The expression level of CHI3L1 is regulated by a variety of factors, including transcription factors, signal transduction pathways, and epigenetic modifications.

What is the association between CHI3L1 and joint diseases such as rheumatoid arthritis?

In joint diseases such as rheumatoid arthritis, the expression level of CHI3L1 may be abnormally elevated, promoting inflammation and joint damage, and aggravating the condition.

How to detect the expression level of CHI3L1?

The expression level of CHI3L1 can be detected by immunohistochemistry, western blotting, and real-time PCR.