Recombinant Human CHMP3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CHMP3-6095H
• Product Overview: VPS24 MS Standard C13 and N15-labeled recombinant protein (NP_057163) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a protein that sorts transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ring finger protein 103 (RNF103) gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 25.1 kDa
• AA Sequence: MGLFGKTQEKPPKELVNEWSLKIRK EMRVVDRQIRDIQREEEKVKRSVKD AAKKGQKDVCIVLAKEMIRSRKAVS KLYASKAHMNSVLMGMKNQLAVLRV AGSLQKSTEVMKAMQSLVKIPEIQA TMRELSKEMMKAGIIEEMLEDTFES MDDQEEMEEEAEMEIDRILFEITAG ALGKAPSKVTDALPEPEPPGAMAAS EDEEEEEEALEAMQSRLATLRSTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CHMP3 charged multivesicular body protein 3 [ Homo sapiens (human) ]
• Official Symbol: CHMP3
• Synonyms: CHMP3; charged multivesicular body protein 3; NEDF; VPS24; CGI-149; charged multivesicular body protein 3; 25.1 protein; CHMP family, member 3; chromatin-modifying protein 3; neuroendocrine differentiation factor; vacuolar protein sorting 24 homolog; vacuolar protein sorting-associated protein 24
• Gene ID: 51652
• mRNA Refseq: NM_016079
• Protein Refseq: NP_057163
• MIM: 610052
• UniProt ID: Q9Y3E7