Recombinant Human CLDN3 293 Cell Lysate

• Cat.No.: CLDN3-7463HCL

Specification

• Description: Antigen standard for claudin 3 (CLDN3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CLDN3 claudin 3 [ Homo sapiens ]
• Official Symbol: CLDN3
• Synonyms: CLDN3; claudin 3; C7orf1, CPETR2; claudin-3; Clostridium perfringens enterotoxin receptor 2; CPE R2; CPE receptor 2; HRVP1; RVP1; ventral prostate.1 like protein; CPE-R 2; CPE-receptor 2; ventral prostate.1-like protein; ventral prostate.1 protein homolog; C7orf1; CPE-R2; CPETR2
• Gene ID: 1365
• mRNA Refseq: NM_001306
• Protein Refseq: NP_001297
• MIM: 602910
• UniProt ID: O15551
• Chromosome Location: 7q11
• Pathway: Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem
• Function: identical protein binding; structural molecule activity; transmembrane signaling receptor activity

Reviews

04/24/2019

The manufacturer's deep understanding of the protein and its applications enables them to promptly and effectively address any issues that may arise during my trials.

06/30/2018

It minimizes the risk of protein degradation and maximizes the effectiveness of the protein in various applications, such as cell signaling pathways or metabolic studies.

12/12/2017

This stability is particularly advantageous for long-term studies or experiments involving extended incubation periods.

Q&As

Is CLDN3 a potential target for drug delivery to specific tissues?

Researchers are exploring the use of CLDN3 as a target for drug delivery systems, aiming to enhance the specificity of drug delivery to tissues expressing high levels of CLDN3.

How does CLDN3 impact the permeability of the intestinal epithelium?

CLDN3 is involved in the tight junctions of intestinal epithelial cells, influencing the permeability of the intestinal barrier and playing a role in conditions like IBD.

In which other diseases or conditions is CLDN3 being investigated?

CLDN3 is under investigation in various conditions, including infectious diseases, where its role in tight junctions may have implications for host-pathogen interactions.

Can CLDN3 be involved in inflammatory bowel diseases (IBD)?

Yes, CLDN3 is implicated in the pathogenesis of inflammatory bowel diseases, and its expression levels are being studied as potential markers for disease severity.

How is CLDN3 linked to neurodegenerative diseases?

Altered expression of CLDN3 in the blood-brain barrier is associated with certain neurodegenerative diseases, making it a subject of interest for researchers studying these conditions.