Recombinant Human COL3A1 Protein, His-tagged

• Cat.No.: COL3A1-2157H
• Product Overview: Recombinant Human COL3A1 Protein (Asp1222-Leu1466) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.2 kDa
  Accurate Molecular Mass: 31 kDa
• Protein length: Asp1222-Leu1466
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: COL3A1 collagen, type III, alpha 1 [ Homo sapiens (human) ]
• Official Symbol: COL3A1
• Synonyms: COL3A1; collagen, type III, alpha 1; EDS4A, Ehlers Danlos syndrome type IV, autosomal dominant; collagen alpha-1(III) chain; collagen, fetal; alpha1 (III) collagen; Ehlers-Danlos syndrome type IV, autosomal dominant; EDS4A; FLJ34534
• Gene ID: 1281
• mRNA Refseq: NM_000090
• Protein Refseq: NP_000081
• MIM: 120180
• UniProt ID: P02461