Recombinant Human COL8A1, His-tagged

• Cat.No.: COL8A1-140H
• Product Overview: Recombinant Human COL8A1(Pro575~Pro743), fused with with N-terminal His-Tag, was expressed in E.coli.

Specification

• Description: This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Molecular Mass: 20.2kDa
• Protein length: Pro575~Pro743
• AA Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.MGHHHHHHSGSEF-PPTPPP QGEYLPDMGL GIDGVKPPHA YGAKKGKNGG PAYEMPAFTA ELTAPFPPVG APVKFNKLLY NGRQNYNPQT GIFTCEVPGV YYFAYHVHCK GGNVWVALFK NNEPVMYTYD EYKKGFLDQA SGSAVLLLRP GDRVFLQMPSEQAAGLYAGQ YVHSSFSGYL LYP
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37ºC for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: COL8A1 collagen, type VIII, alpha 1 [ Homo sapiens (human) ]
• Official Symbol: COL8A1
• Synonyms: COL8A1; collagen, type VIII, alpha 1; C3orf7, chromosome 3 open reading frame 7; collagen alpha-1(VIII) chain; MGC9568; endothelial collagen; collagen VIII, alpha-1 polypeptide; cell proliferation-inducing protein 41; smooth muscle cell-expressed and macrophage conditioned medium-induced protein smag-64; C3orf7
• Gene ID: 1295
• mRNA Refseq: NM_001850
• Protein Refseq: NP_001841
• MIM: 120251
• UniProt ID: P27658
• Chromosome Location: 3q12.3
• Pathway: Assembly of collagen fibrils and other multimeric structures; Collagen biosynthesis and modifying enzymes; Collagen formation; Extracellular matrix organization
• Function: serine-type endopeptidase activity