Recombinant Human CST7 protein(Met1-His145), hFc-tagged

• Cat.No.: CST7-679H
• Product Overview: Recombinant Human CST7 (NP_003641.3) (Met 1-His 145) was expressed in HEK293 with the fused Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-hFc
• Protein length: Met1-His145
• Form: Lyophilized from sterile PBS, pH7. 4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value is <5 nM.
• Molecular Mass: The recombinant human Cystatin-F/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 364 amino acids and predicts a molecular mass of 41.3 kDa. As a result of glycosylation, the rh Cystatin-F/Fc monomer migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CST7 cystatin F (leukocystatin) [ Homo sapiens ]
• Official Symbol: CST7
• Synonyms: CST7; cystatin F (leukocystatin); cystatin-F; cystatin-7; leukocystatin; cystatin-like metastasis-associated protein; CMAP
• Gene ID: 8530
• mRNA Refseq: NM_003650
• Protein Refseq: NP_003641
• MIM: 603253
• UniProt ID: O76096