Recombinant Human CTSL1 protein, His-tagged

• Cat.No.: CTSL1-896H
• Product Overview: Recombinant Human CTSL1 (Thr18-Val333) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Cathepsin L (CTSL1) is also known as major excreted protein (MEP), is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains linked by disulfide bonds. CTSL1 is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. MEP has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. CTSL1 is important for the overall degradation of proteins in lysosomes. The specificity of MEP is close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z - Arg – Arg – NHMec, and no peptidyl - dipeptidase activity. Human Cathepsin L activity is greatest under mildly acidic conditions, from pH 4.5 ­ 6.5. The stability of the enzyme decreases at higher pH values
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Thr18
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM NaAc, 150 mM NaCl, pH5.0, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 25.0 kDa. The protein migrates as 10 kDa and 34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation, corresponding to the propeptide and the mature form respectively.
• Protein length: Thr18-Val333
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >98% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CTSL1
• Official Symbol: CTSL1
• Synonyms: CTSL1; cathepsin L1; cathepsin L , CTSL; FLJ31037; cathepsin L; major excreted protein; MEP; CATL; CTSL
• Gene ID: 1514
• mRNA Refseq: NM_001257971
• Protein Refseq: NP_001244900
• MIM: 116880
• UniProt ID: P07711