Recombinant Human CUL1 protein, His-tagged

• Cat.No.: CUL1-3189H
• Product Overview: Recombinant Human CUL1 protein(111-462 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 111-462 aa
• AA Sequence: YCDSLLKKSSKNPEEAELEDTLNQV MVVFKYIEDKDVFQKFYAKMLAKRL VHQNSASDDAEASMISKLKQACGFE YTSKLQRMFQDIGVSKDLNEQFKKH LTNSEPLDLDFSIQVLSSGSWPFQQ FCTFALPSELERSYQRFTAFYASRH SGRKLTWLYQLSKGELVTNCFKNRY TLQASTFQMAILLQYNTEDAYTVQQ LTDSTQIKMDILAQVLQILLKSKLL VLEDENANVDEVELKPDTLIKLYLG YKNKKLRVNINVPMKTEQKQEQETT HKNIEEDRKLLIQAAIVRIMKMRKV LKHQQLLGEVLTQLSSRFKPRVPVI KKCIDILIEKEYLERVDGEKDTYSY LA
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: CUL1 cullin 1 [ Homo sapiens ]
• Official Symbol: CUL1
• Synonyms: CUL1; cullin 1; cullin-1; CUL-1; MGC149834; MGC149835
• Gene ID: 8454
• mRNA Refseq: NM_003592
• Protein Refseq: NP_003583
• MIM: 603134
• UniProt ID: Q13616