Recombinant Human CXCR4, GST-tagged

• Cat.No.: CXCR4-5138H
• Product Overview: Recombinant Human CXCR4 Protein, With N-GST Tag (rhCXCR4) Met 1 - Ser 46 (Accession # AAH20968.1) was produced in E.coli cells

Specification

• Description: C-X-C chemokine receptor type 4 also known as fusin or CD184 (cluster of differentiation 184), CXCR4, CD184, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R or WHIM. CXCR-4 is an alpha-chemokine receptor specific for stromal-derived-factor-1 (SDF-1 also called CXCL12), a molecule endowed with potent chemotactic activity for lymphocytes. This receptor is one of several chemokine receptors that HIV isolates can use to infect CD4+ T cells. HIV isolates that use CXCR4 are traditionally known as T-cell tropic isolates. Typically, these viruses are found late in infection. It is unclear as to whether the emergence of CXCR4 using HIV is a consequence or a cause of immunodeficiency.CXCR4 is upregulated during the implantation window in natural and hormone replacement therapy cycles in the endometrium, producing, in presence of a human blastocyst, a surface polarization of the CXCR4 receptors suggesting that this receptor is implicated in the adhesion phase of human implantation. SDF-1 and CXCR4 were believed to be a relatively "monogamous" ligand-receptor pair (other chemokines tend to use several different chemokine receptors in a fairly "promiscuous" manner). Recent evidence demonstrates ubiquitin is also a natural ligand of CXCR4. Chronic exposure to THC increased T lymphocyte CXCR4 expression on both CD4+ and CD8+ T lymphocytes. Drugs that block the CXCR4 receptor appear to be capable of "mobilizing" hematopoietic stem cells into the bloodstream as peripheral blood stem cells.
• Source: E. coli
• Species: Human
• Tag: GST
• Predicted N Terminal: Met 1
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with 10 mM reduced glutathione. Normally Mannitol or Trehalose are added as protectants before lyophilization.Contact us for customized product format or formulation.
• Molecular Mass: 32.2 kDa. DTT-reduced Protein migrates as 33 kDa.
• Endotoxin: Less than 1.0 EU per μg of the rhCXCR4 by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).

Gene Information

• Gene Name: CXCR4 chemokine (C-X-C motif) receptor 4 [ Homo sapiens ]
• Official Symbol: CXCR4
• Synonyms: CXCR4; chemokine (C-X-C motif) receptor 4; chemokine (C X C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CD184; D2S201E; fusin; HM89; HSY3RR; LESTR; NPY3R; NPYR; NPYY3R; CXC-R4; CXCR-4; CD184 antigen; SDF-1 receptor; neuropeptide Y receptor Y3; seven transmembrane helix receptor; stromal cell-derived factor 1 receptor; lipopolysaccharide-associated protein 3; seven-transmembrane-segment receptor, spleen; leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; FB22; LAP3; LCR1; WHIM; NPYRL
• Gene ID: 7852
• mRNA Refseq: NM_001008540
• Protein Refseq: NP_001008540
• MIM: 162643
• UniProt ID: P61073
• Chromosome Location: 2q21
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Binding and entry of HIV virion, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem
• Function: C-X-C chemokine receptor activity; G-protein coupled receptor activity; actin binding; coreceptor activity; myosin light chain binding; protein binding; receptor activity; signal transducer activity; ubiquitin binding; ubiquitin protein ligase binding

Reviews

04/07/2022

The responsive customer support team provided quick resolutions to any queries or issues we encountered.

10/28/2021

The product's cost-effectiveness and clear instructions were instrumental in our experimental design.

10/29/2018

We found the product's straightforward protocols highly conducive to seamless integration into our lab work.

Q&As

How does CXCR4 participate in chemotaxis and migration of immune cells, particularly in the context of the immune response?

CXCR4 participates in chemotaxis and migration of immune cells, facilitating their movement toward specific tissues during the immune response.

Are there specific chemokines or ligands that CXCR4 binds to, influencing its role in immune cell activation and recruitment?

Specific chemokines or ligands, such as CXCL12, bind to CXCR4, influencing its role in immune cell activation and recruitment.

In which cellular compartments is CXCR4 predominantly localized, especially within immune cells?

CXCR4 is predominantly localized on the cell surface of immune cells, particularly within the membrane of T cells and monocytes.

What diseases or conditions are associated with dysregulation or abnormal expression of CXCR4, especially in the immune system?

Dysregulation or abnormal expression of CXCR4 is associated with diseases such as cancer, HIV/AIDS, and autoimmune disorders, particularly affecting immune cell function.

Are there therapeutic implications or potential targets related to CXCR4 in diseases involving immune dysregulation and inflammation?

There are potential therapeutic implications related to CXCR4 in diseases involving immune dysregulation and inflammation, making it a target for drug development.

How does CXCR4 contribute to the regulation of inflammatory responses and immune cell trafficking in various tissues?

CXCR4 contributes to the regulation of inflammatory responses and immune cell trafficking in various tissues, influencing the outcome of immune reactions.

What is the primary function of C-X-C chemokine receptor type 4 (CXCR4) in cellular processes and immune responses?

C-X-C chemokine receptor type 4 (CXCR4) plays a crucial role in cellular processes, primarily contributing to immune responses and cell migration.