Recombinant Human CXCR4 protein, His-tagged

• Cat.No.: CXCR4-510H
• Product Overview: Recombinant Human CXCR4 protein (Asp262-Ser352) was fused to His-tag at N-terminus and expressed in E.coli.

Specification

• Description: This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: E.coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 16 kDa as determined by SDS-PAGE reducing conditions.
• Protein length: 91
• AA Sequence: DSFILLEIIKQGCEFENTVHKWISI TEALAFFHCCLNPILYAFLGAKFKT SAQHALTSVSRGSSLKILSKGKRGG HSSVSTESESSSFHSS
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: >90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CXCR4
• Official Symbol: CXCR4
• Synonyms: FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; D2S201E; Fusin; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3
• Gene ID: 7852
• mRNA Refseq: NM_001008540.2
• Protein Refseq: NP_001008540.1
• MIM: 162643
• UniProt ID: P61073

Reviews

04/07/2022

The responsive customer support team provided quick resolutions to any queries or issues we encountered.

10/28/2021

The product's cost-effectiveness and clear instructions were instrumental in our experimental design.

10/29/2018

We found the product's straightforward protocols highly conducive to seamless integration into our lab work.

Q&As

How does CXCR4 participate in chemotaxis and migration of immune cells, particularly in the context of the immune response?

CXCR4 participates in chemotaxis and migration of immune cells, facilitating their movement toward specific tissues during the immune response.

Are there specific chemokines or ligands that CXCR4 binds to, influencing its role in immune cell activation and recruitment?

Specific chemokines or ligands, such as CXCL12, bind to CXCR4, influencing its role in immune cell activation and recruitment.

In which cellular compartments is CXCR4 predominantly localized, especially within immune cells?

CXCR4 is predominantly localized on the cell surface of immune cells, particularly within the membrane of T cells and monocytes.

What diseases or conditions are associated with dysregulation or abnormal expression of CXCR4, especially in the immune system?

Dysregulation or abnormal expression of CXCR4 is associated with diseases such as cancer, HIV/AIDS, and autoimmune disorders, particularly affecting immune cell function.

Are there therapeutic implications or potential targets related to CXCR4 in diseases involving immune dysregulation and inflammation?

There are potential therapeutic implications related to CXCR4 in diseases involving immune dysregulation and inflammation, making it a target for drug development.

How does CXCR4 contribute to the regulation of inflammatory responses and immune cell trafficking in various tissues?

CXCR4 contributes to the regulation of inflammatory responses and immune cell trafficking in various tissues, influencing the outcome of immune reactions.

What is the primary function of C-X-C chemokine receptor type 4 (CXCR4) in cellular processes and immune responses?

C-X-C chemokine receptor type 4 (CXCR4) plays a crucial role in cellular processes, primarily contributing to immune responses and cell migration.