Recombinant Human CYP3A7 protein, His-tagged

• Cat.No.: CYP3A7-1524H
• Product Overview: Recombinant Human CYP3A7 aa. (Pro344~Asp497) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1. Naturally-occurring readthrough transcription occurs between this gene and the downstream CYP3A51P pseudogene and is represented by GeneID:100861540.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Pro344~Asp497
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 9.3

Gene Information

• Gene Name: CYP3A7 cytochrome P450 family 3 subfamily A member 7 [ Homo sapiens (human) ]
• Official Symbol: CYP3A7
• Synonyms: CYP3A7; cytochrome P450 family 3 subfamily A member 7; CP37; CYPIIIA7; P450-HFLA; P-450111A7; P-450(HFL33); cytochrome P450 3A7; aryl hydrocarbon hydroxylase; cytochrome P450, family 3, subfamily A, polypeptide 7; cytochrome P450, subfamily IIIA, polypeptide 7; cytochrome P450-HFLA; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase
• Gene ID: 1551
• mRNA Refseq: NM_000765.4
• Protein Refseq: NP_000756.3
• UniProt ID: P24462