Recombinant Human cytomegalovirus (HCMV) gB protein(Met1-Lys700 & Arg777-Val907), Fc-tagged
Cat.No. : | gB-1045C |
Product Overview : | Recombinant Human CMV gB (AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') (Arg 777-Val 907)(Met 1-Lys 700)precursor was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | CMV |
Tag : | C-Fc |
Protein length : | Met1-Lys700 & Arg777-Val907 |
Form : | Lyophilized from sterile 100mM Gly, 10mM NaCl, 50mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its ability to bind biotinylated Human CD209-Fc in functional ELISA. |
Molecular Mass : | The recombinant human CMV glycoprotein B/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 1045 amino acids and has a predicted molecular mass of 118 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of gB/Fc monomer is approximately 160-170 kDa due to glycosylation. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 80 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Products Types
◆ Lysates | ||
GB-2601CCL | Recombinant CMV GB cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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