Recombinant Human DBT protein(Gly62-Lys482), His-tagged

• Cat.No.: DBT-1075H
• Product Overview: Recombinant Human DBT (NP_001909.3) (Gly62-Lys482) was expressed in Insect Cells with a polyhistidine tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His
• Protein length: Gly62-Lys482
• Form: Lyophilized from sterile 20 mM Tris, pH 8.0, 200 mM NaCl, 20 % glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human DBT consists of 441 amino acids and predicts a molecular mass of 48.8 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DBT dihydrolipoamide branched chain transacylase E2 [ Homo sapiens ]
• Official Symbol: DBT
• Synonyms: DBT; dihydrolipoamide branched chain transacylase E2; dihydrolipoamide branched chain transacylase (E2 component of branched chain keto acid dehydrogenase complex; maple syrup urine disease); lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial; BCKADE2; BCKAD-E2; BCKAD E2 subunit; dihydrolipoyl transacylase; branched chain acyltransferase, E2 component; dihydrolipoyllysine-residue (2-methylpropanoyl)transferase; branched-chain alpha-keto acid dehydrogenase complex component E2; E2 component of branched chain alpha-keto acid dehydrogenase complex; mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit (E2b); dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; lipoamide acyltransferase component of mitochondrial branched-chain alpha-keto acid dehydrogenase complex; E2; E2B; BCATE2; MGC9061
• Gene ID: 1629
• mRNA Refseq: NM_001918
• Protein Refseq: NP_001909
• MIM: 248610
• UniProt ID: P11182