Recombinant Human DCLK2 Protein, His&GST-tagged

• Cat.No.: DCLK2-1318H
• Product Overview: Recombinant Human DCLK2 Protein (Met1-Cys246) with N-His&GST tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified.
• Source: E. coli
• Species: Human
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 57.2 kDa
  Accurate Molecular Mass: 61 kDa
• Protein length: Met1-Cys246
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: DCLK2 doublecortin-like kinase 2 [ Homo sapiens (human) ]
• Official Symbol: DCLK2
• Synonyms: DCLK2; doublecortin-like kinase 2; DCAMKL2, doublecortin and CaM kinase like 2; serine/threonine-protein kinase DCLK2; DCDC3; DCDC3B; DCK2; MGC45428; CaMK-like CREB regulatory kinase 2; doublecortin and CaM kinase-like 2; doublecortin-like and CAM kinase-like 2; doublecortin domain-containing protein 3B; CL2; CLIK2; CLICK2; DCAMKL2; CLICK-II; DKFZp761I032
• Gene ID: 166614
• mRNA Refseq: NM_001040260
• Protein Refseq: NP_001035350
• MIM: 613166
• UniProt ID: Q8N568