Recombinant Human DEK 293 Cell Lysate
Cat.No. : | DEK-6980HCL |
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Description : | Antigen standard for DEK oncogene (DEK), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | DEK DEK oncogene [ Homo sapiens ] |
Official Symbol : | DEK |
Synonyms : | DEK; DEK oncogene; DEK oncogene (DNA binding); protein DEK; D6S231E; |
Gene ID : | 7913 |
mRNA Refseq : | NM_001134709 |
Protein Refseq : | NP_001128181 |
MIM : | 125264 |
UniProt ID : | P35659 |
Chromosome Location : | 6p23 |
Function : | DNA binding; histone binding; |
Products Types
◆ Recombinant Protein | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionEnzyme-linked immunosorbent assay (ELISA) and immunohistochemistry are commonly used to measure DEK protein levels in clinical samples from cancer and autoimmune disease patients.
DEK protein has been shown to play a role in aberrant immune responses, leading to the development of autoimmune diseases such as lupus and rheumatoid arthritis.
DEK protein has been shown to interact with various cellular proteins, modulating signaling pathways and gene expression patterns associated with cancer and autoimmune diseases.
Mutations in the DEK gene and its regulatory regions have been identified in certain cancers, contributing to its aberrant expression and oncogenic potential.
Studies have suggested that DEK protein levels in patient samples may serve as predictive biomarkers for treatment response and resistance in cancer therapy.
Customer Reviews (3)
Write a reviewThis collaboration may extend beyond the purchase of the DEK protein, potentially leading to additional support, joint research projects, or access to expertise in the field.
This may include providing detailed protocols, troubleshooting advice, and suggestions for experimental design.
The manufacturer can provide researchers with high-quality DEK protein, ensuring it is properly purified, characterized, and quality-controlled.
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