Recombinant Human Deoxyuridine Triphosphatase, His-tagged

• Cat.No.: DUT-792H
• Product Overview: Recombinant humanDUT protein,fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 21.6 kDa (204aa), confirmed by MALDI-TOF.

Specification

• Cat. No.: DUT-792H
• Description: DUT, also known as dUTP pyrophosphatase, is a ubiquitous enzyme that functions in nucleotide metabolism. This protein, in the presence of magnesium ions, is responsible for hydrolyzing dUTP to dUMP and diphosphate. This reaction is important for keeping the intracellular dUTP concentration low so that uracil does not become incorporated into DNA. Extensive incorporation of uracil into DNA can ultimately lead to cell death. This suggests that DUT is essential for cell viability, further implying that it is a potential target for anticancer therapy.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MASTVGAAGW KGELPKAGGS PAPGPETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN
• Purity: > 90% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT, 0.1M NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DUT deoxyuridine triphosphatase [ Homo sapiens ]
• Synonyms: DUT; deoxyuridine triphosphatase; dUTPase; FLJ20622; deoxyuridine 5"-triphosphate nucleotidohydrolase, mitochondrial; dUTP pyrophosphatase; dUTP nucleotidohydrolase; EC 3.6.1.23
• Gene ID: 1854
• mRNA Refseq: NM_001025248
• Protein Refseq: NP_001020419
• MIM: 601266
• UniProt ID: P33316
• Chromosome Location: 15q21.1
• Pathway: Metabolic pathways; Pyrimidine metabolism
• Function: dUTP diphosphatase activity; hydrolase activity; protein binding

Reviews

04/01/2021

The DUT protein demonstrates significant relevance and potential in various trial applications, primarily focusing on inflammation, angiogenesis, and tissue remodeling.

04/21/2020

Another advantage of the DUT protein is its reliability and consistency, ensuring robust and reproducible results.

05/12/2017

Whether it is regarding experimental design, optimization of protocols, or troubleshooting issues, their expertise and prompt customer service help researchers overcome challenges efficiently.

Q&As

Can the DUT protein be used as a biomarker in cancer diagnosis?

Yes, alterations in the expression levels of the DUT protein can serve as a biomarker for certain cancers, aiding in early diagnosis and prognosis.

How does the DUT protein contribute to the understanding of neurodegenerative diseases?

Research suggests a link between the DUT protein and neurodegenerative diseases, where its dysfunction may impact DNA repair processes in neurons, potentially contributing to disease progression.

Are there any specific cancer types where the DUT protein is particularly relevant?

Research suggests that the DUT protein is particularly relevant in colorectal cancer, where its abnormal expression may impact DNA replication and repair mechanisms.

How is the DUT protein clinically significant in cancer research?

The DUT protein is implicated in cancer research as its dysregulation can lead to an imbalance in nucleotide pools, potentially contributing to genomic instability, a common feature in cancer cells.

How does the DUT protein contribute to the development of targeted therapies in cancer treatment?

Understanding the role of the DUT protein helps in developing targeted therapies that aim to modulate its activity, potentially disrupting cancer cell proliferation.