Recombinant Human DFFA, His-tagged

• Cat.No.: DFFA-27566TH
• Product Overview: ICAD recombinant full length protein (Human), with an N-terminal His-tag, ~48kDa.

Specification

• Description: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Conjugation: HIS
• Source: E. coli
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 50% Glycerol, PBS
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequence Similarities: Contains 1 CIDE-N domain.

Gene Information

• Gene Name: DFFA DNA fragmentation factor, 45kDa, alpha polypeptide [ Homo sapiens ]
• Official Symbol: DFFA
• Synonyms: DFFA; DNA fragmentation factor, 45kDa, alpha polypeptide; DNA fragmentation factor, 45 kD, alpha polypeptide; DNA fragmentation factor subunit alpha; DFF 45; DFF1; DFF45; DNA fragmentation factor; 45 kD; alpha subunit; ICAD
• Gene ID: 1676
• mRNA Refseq: NM_004401
• Protein Refseq: NP_004392
• MIM: 601882
• Uniprot ID: O00273
• Chromosome Location: 1p36.3-p36.2
• Pathway: Activation of DNA fragmentation factor, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem
• Function: deoxyribonuclease activity

Reviews

12/23/2021

We felt well-supported throughout our usage of the product, thanks to the dedicated customer service.

10/18/2019

Consistency in the product's behavior across experimental conditions enhanced the credibility of our findings.

05/11/2019

The product's adaptability to various buffers contributed to its stable and reliable performance.

Q&As

What diseases or conditions are associated with dysregulation or abnormal expression of DFFA, particularly in the context of apoptotic dysfunction?

Dysregulation or abnormal expression of DFFA is associated with various conditions, including cancer and neurodegenerative disorders, highlighting its role in apoptotic dysfunction.

How does DFFA contribute to DNA fragmentation and chromatin condensation during apoptotic cell death?

DFFA contributes to DNA fragmentation and chromatin condensation by being cleaved and activated by caspases, particularly caspase-3, during apoptotic cell death.

In which cellular compartments is DFFA predominantly localized, especially during the initiation of apoptosis?

DFFA is predominantly localized in the nucleus, specifically during the initiation of apoptosis, where it actively participates in chromatin condensation and DNA fragmentation.

Are there therapeutic implications or potential targets related to DFFA in diseases involving apoptotic dysregulation and abnormal cell survival?

There are potential therapeutic implications related to DFFA in diseases involving apoptotic dysregulation and abnormal cell survival, making it a target for drug development.

What is the primary function of DNA Fragmentation Factor Subunit Alpha (DFFA) in cellular processes and apoptosis?

DNA Fragmentation Factor Subunit Alpha (DFFA) plays a crucial role in cellular processes, primarily contributing to the execution of apoptosis and programmed cell death.

How does DFFA participate in regulating the balance between cell survival and programmed cell death in various tissues?

DFFA actively participates in regulating the balance between cell survival and programmed cell death in various tissues, influencing tissue homeostasis.

Are there specific caspases or factors that activate DFFA, influencing its role in the execution phase of apoptosis?

Specific caspases, including caspase-3, activate DFFA, influencing its role in the execution phase of apoptosis and facilitating efficient DNA degradation.