Recombinant Human DFFA Protein, Myc/DDK-tagged, C13 and N15-labeled
Cat.No. : | DFFA-4460H |
Product Overview : | DFFA MS Standard C13 and N15-labeled recombinant protein (NP_004392) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
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Description : | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
Source : | HEK293 |
Species : | Human |
Tag : | Myc/DDK |
Molecular Mass : | 36.6 kDa |
AA Sequence : | MEVTGDAGVPESGEIRTLKPCLLRR NYSREQHGVAASCLEDLRSKACDIL AIDKSLTPVTLVLAEDGTIVDDDDY FLCLPSNTKFVALASNEKWAYNNSD GGTAWISQESFDVDETDSGAGLKWK NVARQLKEDLSSIILLSEEDLQMLV DAPCSDLAQELRQSCATVQRLQHTL QQVLDQREEVRQSKQLLQLYLQALE KEGSLLSKQEESKAAFGEEVDAVDT GISRETSSDVALASHILTALREKQA PELSLSSQDLELVTKEDPKALAVAL NWDIKKTETVQEACEWELALRLQQT QSLHSLRSISASKASPPGDLQNPKR ARQDPTTRTRPLEQKLISEEDLAAN DILDYKDDDDKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : | Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : | 50 μg/mL as determined by BCA |
Storage Buffer : | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
Gene Name : | DFFA DNA fragmentation factor subunit alpha [ Homo sapiens (human) ] |
Official Symbol : | DFFA |
Synonyms : | DFFA; DNA fragmentation factor, 45kDa, alpha polypeptide; DNA fragmentation factor, 45 kD, alpha polypeptide; DNA fragmentation factor subunit alpha; DFF 45; DFF1; DFF45; DNA fragmentation factor; 45 kD; alpha subunit; ICAD; inhibitor of CAD; DNA fragmentation factor 45 kDa subunit; DFF-45; |
Gene ID : | 1676 |
mRNA Refseq : | NM_004401 |
Protein Refseq : | NP_004392 |
MIM : | 601882 |
UniProt ID : | O00273 |
Products Types
◆ Recombinant Protein | ||
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DFFA-1074R | Recombinant Rhesus Macaque DFFA Protein, His (Fc)-Avi-tagged | +Inquiry |
Dffa-2532M | Recombinant Mouse Dffa Protein, Myc/DDK-tagged | +Inquiry |
DFFA-2341M | Recombinant Mouse DFFA Protein, His (Fc)-Avi-tagged | +Inquiry |
Dffa-1836M | Recombinant Mouse Dffa protein, His & T7-tagged | +Inquiry |
◆ Lysates | ||
DFFA-6967HCL | Recombinant Human DFFA 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionDysregulation or abnormal expression of DFFA is associated with various conditions, including cancer and neurodegenerative disorders, highlighting its role in apoptotic dysfunction.
DFFA contributes to DNA fragmentation and chromatin condensation by being cleaved and activated by caspases, particularly caspase-3, during apoptotic cell death.
DFFA is predominantly localized in the nucleus, specifically during the initiation of apoptosis, where it actively participates in chromatin condensation and DNA fragmentation.
There are potential therapeutic implications related to DFFA in diseases involving apoptotic dysregulation and abnormal cell survival, making it a target for drug development.
DNA Fragmentation Factor Subunit Alpha (DFFA) plays a crucial role in cellular processes, primarily contributing to the execution of apoptosis and programmed cell death.
DFFA actively participates in regulating the balance between cell survival and programmed cell death in various tissues, influencing tissue homeostasis.
Specific caspases, including caspase-3, activate DFFA, influencing its role in the execution phase of apoptosis and facilitating efficient DNA degradation.
Customer Reviews (3)
Write a reviewWe felt well-supported throughout our usage of the product, thanks to the dedicated customer service.
Consistency in the product's behavior across experimental conditions enhanced the credibility of our findings.
The product's adaptability to various buffers contributed to its stable and reliable performance.
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