Recombinant Human DGKE protein, His-tagged
Cat.No. : | DGKE-597H |
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Product overview : | Recombinant Human DGKE (P52429) aa. (Leu243~Gly502) fused with His tag was produced in E. coli cells. |
Description : | Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Freeze-dried powder |
Molecular Mass : | Predicted Molecular Mass: 32.5kDa; Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions. |
Protein length : | Leu243~Gly502 |
Purity : | >95% |
Characteristic : | Isoelectric point is 7.7 . |
Applications : | Cell culture; Activity Assays; In vivo assays. |
Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage buffer : | 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose. |
Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex |
Gene Name : | DGKE diacylglycerol kinase epsilon [ Homo sapiens (human) ] |
Official Symbol : | DGKE |
Synonyms : | DGKE; diacylglycerol kinase epsilon; DGK; AHUS7; DAGK5; DAGK6; NPHS7; DAG kinase epsilon; DGK-epsilon; diacylglycerol kinase, epsilon 64kDa; diglyceride kinase epsilon; |
Gene ID : | 8526 |
mRNA Refseq : | NM_003647.2 |
Protein Refseq : | NP_003638.1 |
UniProt ID : | P52429 |
Products Types
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◆ Lysates | ||
DGKE-6957HCL | Recombinant Human DGKE 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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