Recombinant Human DHPS, GST-tagged

• Cat.No.: DHPS-11970H
• Product Overview: Recombinant Human DHPS protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: 1-369a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: DHPS deoxyhypusine synthase [ Homo sapiens ]
• Official Symbol: DHPS
• Synonyms: DHPS; deoxyhypusine synthase; MIG13; migration inducing gene 13; migration-inducing gene 13; DS; DHS
• Gene ID: 1725
• mRNA Refseq: NM_001206974
• Protein Refseq: NP_001193903
• MIM: 600944
• UniProt ID: P49366
• Chromosome Location: 19p13.2
• Pathway: Hypusine synthesis from eIF5A-lysine, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; PTM: gamma carboxylation, hypusine formation and arylsulfatase activation, organism-specific biosystem; Post-translational protein modification, organism-specific biosystem; hypusine biosynthesis, organism-specific biosystem; hypusine biosynthesis, conserved biosystem
• Function: deoxyhypusine synthase activity; protein binding; transferase activity

Reviews

12/26/2020

Their expert team has been instrumental in addressing any challenges or concerns I have encountered during my experiments.

02/14/2019

Their prompt and comprehensive assistance has proven invaluable in overcoming obstacles and optimizing my experimental protocols.

02/05/2019

The DHPS protein boasts remarkable stability, allowing for extended storage without compromising its integrity or performance.

Q&As

Are there any resistance mechanisms against sulfonamides targeting DHPS?

Yes, bacterial resistance to sulfonamides can arise due to mutations in DHPS that reduce the drug's binding affinity.

Can DHPS mutations affect the effectiveness of sulfonamides in treating bacterial infections?

Yes, mutations in DHPS can lead to reduced effectiveness of sulfonamides, contributing to antibiotic resistance.

Are there any other clinical implications of DHPS beyond antibiotic treatment?

DHPS mutations have been associated with resistance to sulfadoxine-pyrimethamine, a drug used to prevent and treat malaria.

In what clinical conditions are sulfonamides commonly used?

Sulfonamides are used to treat urinary tract infections, respiratory tract infections, and other bacterial infections.

Can DHPS be a target for drug development beyond antibiotics?

DHPS has potential as a target for developing new antiparasitic drugs due to its role in folate biosynthesis in parasites such as Plasmodium.