Recombinant Human DIO3 protein, His-tagged

• Cat.No.: DIO3-3713H
• Product Overview: Recombinant Human DIO3 protein(192-278 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 192-278 aa
• AA Sequence: QHRSLEDRVSAARVLQQGAPGCALV LDTMANSSSSAYGAYFERLYVIQSG TIMYQGGRGPDGYQVSELRTWLERY DEQLHGARPRRV
• Purity: 98%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: DIO3 deiodinase, iodothyronine, type III [ Homo sapiens ]
• Official Symbol: DIO3
• Synonyms: DIO3; deiodinase, iodothyronine, type III; TXDI3; type III iodothyronine deiodinase; type 3 DI; type-III 5 deiodinase; type-III 5-deiodinase; type 3 iodothyronine selenodeiodinase; iodothyronine deiodinase, placental type; thyroxine deiodinase type III (selenoprotein); D3; 5DIII; DIOIII
• Gene ID: 1735
• mRNA Refseq: NM_001362
• Protein Refseq: NP_001353
• MIM: 601038
• UniProt ID: P55073