Recombinant Human DLAT Protein (93-640 aa), His-tagged

• Cat.No.: DLAT-456H
• Product Overview: Recombinant Human DLAT Protein (93-640 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Metabolism. Protein Description: Partial.

Specification

• Description: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 62.2 kDa
• Protein length: 93-640 aa
• AA Sequence: KVPLPSLSPTMQAGTIARWEKKEGD KINEGDLIAEVETDKATVGFESLEE CYMAKILVAEGTRDVPIGAIICITV GKPEDIEAFKNYTLDSSAAPTPQAA PAPTPAATASPPTPSAQAPGSSYPP HMQVLLPALSPTMTMGTVQRWEKKV GEKLSEGDLLAEIETDKATIGFEVQ EEGYLAKILVPEGTRDVPLGTPLCI IVEKEADISAFADYRPTEVTDLKPQ VPPPTPPPVAAVPPTPQPLAPTPSA PCPATPAGPKGRVFVSPLAKKLAVE KGIDLTQVKGTGPDGRITKKDIDSF VPSKVAPAPAAVVPPTGPGMAPVPT GVFTDIPISNIRRVIAQRLMQSKQT IPHYYLSIDVNMGEVLLVRKELNKI LEGRSKISVNDFIIKASALACLKVP EANSSWMDTVIRQNHVVDVSVAVST PAGLITPIVFNAHIKGVETIANDVV SLATKAREGKLQPHEFQGGTFTISN LGMFGIKNFSAIINPPQACILAIGA SEDKLVPADNEKGFDVASMMSVTLS CDHRVVDGAVGAQWLAEFRKYLE
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: DLAT dihydrolipoamide S-acetyltransferase [ Homo sapiens ]
• Official Symbol: DLAT
• Synonyms: DLAT; DLTA; PDC E2; PBC; PDCE2; PDC-E2
• Gene ID: 1737
• mRNA Refseq: NM_001931
• Protein Refseq: NP_001922
• MIM: 608770
• UniProt ID: P10515