Recombinant Human DNA Fragmentation Factor, 45kDa, Alpha Polypeptide, His-tagged
Cat.No. : | DFFA-4316H |
Product Overview : | DFFA Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 351 amino acids (1- 331 a.a.) and having a molecular mass of 38.7kDa.The DFFA is purified by proprietary chromatographic techniques. |
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Cat. No. : | DFFA-4316H |
Description : | DFF is a heterodimeric protein of 40kDa (DFFB) and 45kDa (DFFA) subunits. DFFA (DNA fragmentation factor subunit alpha) is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF is activated once DFFA is cleaved by caspase-3. The cleaved fragments of DFFA detach from DFFB (the active component of DFF), which in turn triggers DNA fragmentation as well as chromatin condensation during apoptosis. Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. A reduced level of DFFA detected in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression. DFFA at chromosome 1 shows rare allelic variants in neuroblastoma tumors. |
Source : | Human |
Host : | Escherichia Coli. |
Form : | The DFFA solution contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1mM DTT. |
Purity : | Greater than 90.0% as determined by SDS-PAGE. |
Physical Appearance : | Sterile Filtered colorless solution. |
Amino acid sequence : | MGSSHHHHHH SSGLVPRGSH MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE WELALRLQQT QSLHSLRSIS ASKASPPGDL QNPKRARQDP T. |
Storage : | DFFA although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Pathways : | Apoptosis |
Gene Name : | DFFA DNA fragmentation factor, 45kDa, alpha polypeptide [ Homo sapiens ] |
Official Symbol : | DFFA |
Synonyms : | DFFA; DNA fragmentation factor, 45kDa, alpha polypeptide; DFF1; ICAD; DFF-45; DFF45; ICAD; DFF1; DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; Inhibitor of CAD; DFF45; H13; OTTHUMP00000001903; OTTHUMP00000001904; DNA fragmentation factor, 45 kD, alpha subunit |
Gene ID : | 1676 |
mRNA Refseq : | NM_004401 |
Protein Refseq : | NP_004392 |
MIM : | 601882 |
UniProt ID : | O00273 |
Chromosome Location : | 1p36.3-p36.2 |
Function : | caspase-activated deoxyribonuclease activity; identical protein binding |
Products Types
◆ Recombinant Protein | ||
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Dffa-2532M | Recombinant Mouse Dffa Protein, Myc/DDK-tagged | +Inquiry |
DFFA-2555H | Recombinant Human DFFA Protein, GST-tagged | +Inquiry |
DFFA-1955H | Recombinant Human DFFA Protein (Ala51-Ser305), N-His tagged | +Inquiry |
◆ Lysates | ||
DFFA-6967HCL | Recombinant Human DFFA 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionDysregulation or abnormal expression of DFFA is associated with various conditions, including cancer and neurodegenerative disorders, highlighting its role in apoptotic dysfunction.
DFFA contributes to DNA fragmentation and chromatin condensation by being cleaved and activated by caspases, particularly caspase-3, during apoptotic cell death.
DFFA is predominantly localized in the nucleus, specifically during the initiation of apoptosis, where it actively participates in chromatin condensation and DNA fragmentation.
There are potential therapeutic implications related to DFFA in diseases involving apoptotic dysregulation and abnormal cell survival, making it a target for drug development.
DNA Fragmentation Factor Subunit Alpha (DFFA) plays a crucial role in cellular processes, primarily contributing to the execution of apoptosis and programmed cell death.
DFFA actively participates in regulating the balance between cell survival and programmed cell death in various tissues, influencing tissue homeostasis.
Specific caspases, including caspase-3, activate DFFA, influencing its role in the execution phase of apoptosis and facilitating efficient DNA degradation.
Customer Reviews (3)
Write a reviewWe felt well-supported throughout our usage of the product, thanks to the dedicated customer service.
Consistency in the product's behavior across experimental conditions enhanced the credibility of our findings.
The product's adaptability to various buffers contributed to its stable and reliable performance.
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