Recombinant Human DnaJ (Hsp40) Homolog, Subfamily B, Member 1, GST-tagged

• Cat.No.: DNAJB1-4839H
• Product Overview: Recombinant full-length human DNAJB1 was expressed in E. coli cells using an N-terminal GST tag.

Specification

• Cat. No.: DNAJB1-4839H
• Description: DNAJB1 is a member of heat shock proteins (HSPs) which are synthesized in cells in response to heat shock and other metabolic stresses and provide a transient state of thermotolerance. DNAJB1 is localized faintly throughout the cell in non-heat-shocked cells and accumulates in the nuclei and nucleoli in heat-shocked cells. The intracellular localization of DNAJB1 is very similar to that of HSP70. Overexpression of HSP40 reduces aggregate formation and suppresses apoptosis in a neuronal cell model of spinal and bulbar muscular atrophy (inherited neurodegenerative diseases caused by polyglutamine expansion).
• Applications: Western Blot
• Molecular Weight: 65 kDa
• Expression System: E.coli
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, ph 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Purity: > 90 %
• Concentration: 0.2 ug/ul
• Sequences: Full length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Influenza A; Protein processing in endoplasmic reticulum

Gene Information

• Gene Name: DNAJB1 DnaJ (Hsp40) homolog, subfamily B, member 1 [ Homo sapiens ]
• Official Symbol: DNAJB1
• Synonyms: DNAJB1; DnaJ (Hsp40) homolog, subfamily B, member 1; Hdj1; Sis1; HSPF1; Hsp40; DNAJ1; HDJ-1; HDJ1; DnaJ protein homolog 1; Heat shock protein 40; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; DnaJ (Hsp40) homolog, subfamily B, member 1; Hsp40 (heat shock protein 40 kD)
• Gene ID: 3337
• mRNA Refseq: NM_006145
• Protein Refseq: NP_006136
• MIM: 604572
• UniProt ID: P25685
• Chromosome Location: 19p13.2
• Function: Heat shock protein binding; Unfolded protein binding

Reviews

07/15/2022

Efficient peptide synthesis, crucial for advancing our peptide research.

07/21/2021

Exceptional protein engineering services, a vital resource for our lab.

11/26/2020

Reliable protein crystallography, a crucial part of structural research.

Q&As

How does DNAJB1 contribute to the pathogenesis of neurodegenerative diseases, such as Alzheimer's and Parkinson's?

DNAJB1 plays a role in neurodegenerative diseases by influencing the handling of misfolded proteins in neurons.

How does DNAJB1 influence the immune response, especially in regulating inflammation and immune cell function?

DNAJB1 modulates the immune system, particularly in controlling inflammation and immune cell responses.

How does DNAJB1 assist in protein folding and prevent misfolding or aggregation of proteins within cells?

DNAJB1 aids in protein folding and prevents the aggregation of misfolded proteins, crucial for maintaining cellular proteostasis.

What are the mechanisms by which DNAJB1 modulates apoptosis and cell survival under stress?

DNAJB1 regulates apoptosis and cell survival, especially under conditions of cellular stress.

What is the impact of DNAJB1 on cancer cell survival, proliferation, and response to chemotherapy?

DNAJB1 affects cancer cell behavior, influencing tumor growth, survival, and sensitivity to treatments.

What role does DNAJB1 play in cellular responses to heat shock and other stress conditions?

DNAJB1 is key in cellular stress responses, particularly to heat shock, by stabilizing proteins and aiding in recovery.

How do alterations in DNAJB1 expression or function affect muscle cell health and the development of muscular disorders?

Changes in DNAJB1 expression impact muscle cell function and health, potentially contributing to muscular disorders.