Recombinant Human DnaJ (Hsp40) Homolog, Subfamily B, Member 1, His-tagged

• Cat.No.: DNAJB1-4996H
• Product Overview: Recombinant human HSP40, his-tagged, is overexpressed in E. coli and purified by using the conventional column chromatography techniques.

Specification

• Cat. No.: DNAJB1-4996H
• Description: Human HSP40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. HSP40 protein contains the J-domain which controls interaction with the ATPase domain of DnaK.
• Concentration: 1 mg/ml
• Source: Human
• Host: E. coli
• Form: Supplied as a liquid in 20mM Tris, pH 8.0, containing 2mM DTT, 0.2mM PMSF, and 10% glycerol.
• Purity: > 95% by SDS - PAGE
• Sequence: MGSSHHHHHH SSGLVPRGSH MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE DKILTIEVKK GW
• Molecular Mass: 40.2 kDa (360aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Endotoxin Levels: < 1.0 EU per ug of protein (determined by LAL method)
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: DNAJB1 DnaJ (Hsp40) homolog, subfamily B, member 1 [ Homo sapiens ]
• Official Symbol: DNAJB1
• Synonyms: DNAJB1; DnaJ (Hsp40) homolog, subfamily B, member 1; Hdj1; Sis1; HSPF1; Hsp40; DNAJ1; HDJ-1; HDJ1; DnaJ protein homolog 1; Heat shock protein 40; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; DnaJ (Hsp40) homolog, subfamily B, member 1; Hsp40 (heat shock protein 40 kD)
• Gene ID: 3337
• mRNA Refseq: NM_006145
• Protein Refseq: NP_006136
• MIM: 604572
• UniProt ID: P25685
• Chromosome Location: 19p13.2
• Function: heat shock protein binding; unfolded protein binding

Reviews

07/15/2022

Efficient peptide synthesis, crucial for advancing our peptide research.

07/21/2021

Exceptional protein engineering services, a vital resource for our lab.

11/26/2020

Reliable protein crystallography, a crucial part of structural research.

Q&As

How does DNAJB1 contribute to the pathogenesis of neurodegenerative diseases, such as Alzheimer's and Parkinson's?

DNAJB1 plays a role in neurodegenerative diseases by influencing the handling of misfolded proteins in neurons.

How does DNAJB1 influence the immune response, especially in regulating inflammation and immune cell function?

DNAJB1 modulates the immune system, particularly in controlling inflammation and immune cell responses.

How does DNAJB1 assist in protein folding and prevent misfolding or aggregation of proteins within cells?

DNAJB1 aids in protein folding and prevents the aggregation of misfolded proteins, crucial for maintaining cellular proteostasis.

What are the mechanisms by which DNAJB1 modulates apoptosis and cell survival under stress?

DNAJB1 regulates apoptosis and cell survival, especially under conditions of cellular stress.

What is the impact of DNAJB1 on cancer cell survival, proliferation, and response to chemotherapy?

DNAJB1 affects cancer cell behavior, influencing tumor growth, survival, and sensitivity to treatments.

What role does DNAJB1 play in cellular responses to heat shock and other stress conditions?

DNAJB1 is key in cellular stress responses, particularly to heat shock, by stabilizing proteins and aiding in recovery.

How do alterations in DNAJB1 expression or function affect muscle cell health and the development of muscular disorders?

Changes in DNAJB1 expression impact muscle cell function and health, potentially contributing to muscular disorders.