Recombinant Human DNASE1 Protein (Gly19-Ala259), His tagged

• Cat.No.: DNASE1-1974H
• Product Overview: Recombinant human DNASE1 (Gly19-Ala259) fused with His tag was expressed in E. coli.

Specification

• Description: Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 27.9 kDa
• Protein Length: Gly19-Ala259
• Purity: >90% as determined by SDS-PAGE
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS, pH 7.5 or lyophilized from PBS, pH 7.5.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: DNASE1 deoxyribonuclease I [ Homo sapiens (human) ]
• Official Symbol: DNASE1
• Synonyms: DNASE1; deoxyribonuclease I; DNL1; deoxyribonuclease-1; Dornase alfa; DNase I, lysosomal; human urine deoxyribonuclease I; DRNI; FLJ38093; FLJ44902; DKFZp686H0155
• Gene ID: 1773
• mRNA Refseq: NM_005223
• Protein Refseq: NP_005214
• MIM: 125505
• UniProt ID: P24855

Reviews

12/06/2022

Dependable antibody validation, vital for our immunoprecipitation studies.

01/10/2022

Top-quality protein purification service, essential for our lab's work.

02/22/2019

Fast and reliable protein expression, a critical part of our projects.

Q&As

How does DNASE1 (Deoxyribonuclease I) contribute to DNA degradation and clearance, particularly in apoptotic cells?

DNASE1 helps degrade DNA in apoptotic cells, ensuring proper clearance and preventing inflammation.

How does DNASE1 deficiency lead to the development of systemic lupus erythematosus (SLE) and other autoimmune diseases?

DNASE1 deficiency can contribute to autoimmune diseases like SLE by allowing self-DNA accumulation.

What is the impact of DNASE1 on the regulation of inflammation and immune system activation?

DNASE1 regulates inflammation and immune responses by controlling extracellular DNA levels.

How does DNASE1 influence the resolution of inflammation and tissue repair processes?

DNASE1 aids in resolving inflammation and promoting tissue repair by degrading DNA in damaged tissues.

What are the mechanisms by which DNASE1 modulates the stability and integrity of extracellular DNA?

DNASE1 maintains the stability and integrity of extracellular DNA, preventing inappropriate immune activation.

How do alterations in DNASE1 expression or activity affect susceptibility to chronic inflammatory conditions?

Changes in DNASE1 function can increase susceptibility to chronic inflammation and autoimmune disorders.

What role does DNASE1 play in preventing the accumulation of self-DNA and autoimmune responses?

DNASE1 prevents the accumulation of self-DNA, reducing the risk of autoimmune reactions.